Substrate:LAMP2a:HSP90 polymerizes

Stable Identifier
R-HSA-9624158
Type
Reaction [transition]
Species
Homo sapiens
Compartment
lysosomal lumen, lysosomal membrane
ReviewStatus
5/5
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Substrate:LAMP2a:HSP90 polymerizes
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Intracellular proteins are targeted for proteolytic degradation in the lysosome with the aid of chaperones. Heat shock cognate 71 kDa protein (HSPA8) acts as the constitutive chaperone that binds KFERQ-domain containing substrates in the cytosol. Consequently, the Hspa8:Substrate complex translocates from cytosol to lysosomal membrane where it binds to Lysosome-associated membrane glycoprotein 2 (LAMP2a). Subsequently, HSPA8 is released and Heat shock protein HSP 90 binds to the lysosomal luminal end of LAMP2a. Binding of HSP90 stabilizes LAMP2 to multimerize into a 700 kDa complex (Bandyopadhyay U et al. 2008). This facilitates the internalization of substrate into the lumen. Experiments confirming this binding were performed on rat models.
Literature References
PubMed ID Title Journal Year
18644871 The chaperone-mediated autophagy receptor organizes in dynamic protein complexes at the lysosomal membrane

Cuervo, AM, Kaushik, S, Bandyopadhyay, U, Varticovski, L

Mol. Cell. Biol. 2008
20797626 Identification of regulators of chaperone-mediated autophagy

Cuervo, AM, Kiffin, R, Sridhar, S, Kaushik, S, Bandyopadhyay, U

Mol. Cell 2010
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Substrate:Lamp2a:Hsp90 polymerizes (Rattus norvegicus)
Authored
Varusai, TM  (2019-02-21)
Reviewed
Metzakopian, E  (2019-02-22)
Created
Varusai, TM  (2018-10-08)
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