Cholesterol-loading or treatment with the synthetic agonists of liver X-receptors alpha (LXRα, NR1H3) and beta (LXRβ, NR1H2), such as T0901317 or GW3965, significantly induced the expression of ADP-ribosylation factor-like 4C (ARL4C or ARL7) in murine RAW 264.7 and human THP1 macrophage cell lines (Hong C et al. 2011; Engel T et al. 2004; Sun D et al. 2012). Similar regulation of ARL4C mRNA expression was observed in human peripheral blood-derived monocytes (Hong C et al. 2011). Sequence analysis of the ARL4C 5'-flanking region identified two LXR response elements (LXRE) containing variant direct repeats with a 4 nucleotide spacer (DR4) at –1405 bp and −5215 bp relative to the transcription start site (Hong C et al. 2011). Electrophoretic mobility shift assay showed that LXR:RXR heterodimers efficiently bind the putative ARL4C LXREs to regulate transcription from the promoter (Hong C et al. 2011).
Salazar, JV, Marathe, C, Dhamko, H, Walczak, R, Hong, C, Bradley, MN, Tontonoz, P, Boyadjian, R
Rust, S, Hobohm, U, Schlueter, B, Seedorf, U, Pech, M, Assmann, G, Lorkowski, S, Cullen, P, Engel, T, Bode, G, Lueken, A
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