Reactome | MPST transfers sulfur atom from 3MPYR to form CysS248-MPST

MPST transfers sulfur atom from 3MPYR to form CysS248-MPST

Stable Identifier

R-HSA-9034756

Type

Reaction [transition]

Species

Homo sapiens

Compartment

mitochondrial matrix

ReviewStatus

5/5

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MPST transfers sulfur atom from 3MPYR to form CysS248-MPST

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Hydrogen polysulfides (H₂Sn, where n>=2) are also endogenously produced by 3-mercaptopyruvate sulfurtransferase (MPST aka 3MST) directly from 3-mercaptopyruvate (3MPYR) generated by cysteine (aspartate) aminotransferase (GOT2) (Kimura et al. 2013, Kimura et al. 2015, Koike et al. 2017). Where n=2, the H₂S₂ species is called hydrogen persulfide (aka disulfane). MPST can release either H₂S or H₂Sn depending on the interaction with thioredoxin. When there is strong interaction with thioredoxin, H₂S is released. 3MST receives sulfur from 3MPYR to persulfurate (oxidise) cysteine-248 residue of its reaction centre.

H₂S₂ is the dominant form produced with H₂S₃ detected at lower concentrations in cells or tissues. Up to H₂S₃₅ may exist (Steudel 2003), but under physiological conditions, when n reaches 8, it forms a crown shape and precipitates. H₂Sn activate transient receptor potential ankyrin 1 (TRPA1) channels (Kimura et al. 2013), facilitate translocation of nuclear factor like-2 (NRF2) to the nucleus by modifying its binding partner kelch-like ECH-associated protein 1 (KEAP1) (Koike et al. 2013), regulates the activity of the tumor suppressor phosphatase and tensin homolog (PTEN) (Greiner et al. 2013), and reduces blood pressure by activating protein kinase G1a (Stubbert et al. 2014). Another persulfurated molecule, cysteine persulfide, which may be involved in the regulation of cellular redox homeostasis, is also produced by MPST (Kimura et al. 2017).

Literature References

PubMed ID	Title	Journal	Year
26437775	Identification of H2S3 and H2S produced by 3-mercaptopyruvate sulfurtransferase in the brain Toyofuku, Y, Koike, S, Kimura, Y, Ogasawara, Y, Shibuya, N, Kimura, H, Nagahara, N, Lefer, D	Sci Rep	2015
23413359	Polysulfides are possible H2S-derived signaling molecules in rat brain Tsugane, M, Kimura, Y, Oka, J, Mikami, Y, Kimura, H, Osumi, K	FASEB J.	2013
27484215	Hydrogen polysulfide (H2S n ) signaling along with hydrogen sulfide (H2S) and nitric oxide (NO) Kimura, H	J Neural Transm (Vienna)	2016
29055825	Analysis of endogenous H2S and H2Sn in mouse brain by high-performance liquid chromatography with fluorescence and tandem mass spectrometric detection Kimura, Y, Kawamura, K, Koike, S, Ogasawara, Y, Shibuya, N, Kimura, H	Free Radic. Biol. Med.	2017