Reactome | sABO-A:Mn2+ transfers GalNAc to H antigen-sec to form A antigen-sec

sABO-A:Mn2+ transfers GalNAc to H antigen-sec to form A antigen-sec

Stable Identifier

R-HSA-9034042

Type

Reaction [transition]

Species

Homo sapiens

Compartment

extracellular region

ReviewStatus

5/5

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sABO-A:Mn2+ transfers GalNAc to H antigen-sec to form A antigen-sec

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As well as being a Golgi membrane resident, the histo-blood group ABO system transferase (ABO) can be proteolytically processed by an unknown protease into a soluble form, fucosylglycoprotein alpha-N-acetylgalactosaminyltransferase (sABO). A, B and AB individuals express glycosyltransferase activities that convert the H antigen to the A antigen (by addition of GalNAc), to the B antigen (by addition of Gal) or to the AB antigen (by the addition of both GalNAc and Gal). O group individuals lack such activity. Differences in four critical amino acids (176, 235, 266 and 268) alter the specificity from an A to a B glycosyltransferase (Yamamoto et al. 1990, Yamamoto & McNeill 1996, Seto et al. 1999, Alfaro et al. 2008). The soluble form of histo-blood group A transferase (sABO-A) utilises UDP-GalNAc to transfer N-acetylgalactosamine (GalNAc) to the H antigen formed via Type 1 chains to form the A antigen in secretors (A antigen-sec) (Patenaude et al. 2002, Persson et al. 2007).

Literature References

PubMed ID	Title	Journal	Year
2333095	Molecular genetic basis of the histo-blood group ABO system Marken, J, White, T, Yamamoto, F, Hakomori, S, Clausen, H	Nature	1990
18192272	ABO(H) blood group A and B glycosyltransferases recognize substrate via specific conformational changes Seto, NO, Bai, Y, Borisova, SN, Persson, M, Alfaro, JA, Evans, SV, Polakowski, R, Letts, JA, Zheng, RB, Palcic, MM, Lowary, TL	J. Biol. Chem.	2008
8631849	Amino acid residue at codon 268 determines both activity and nucleotide-sugar donor substrate specificity of human histo-blood group A and B transferases. In vitro mutagenesis study Yamamoto, F, McNeill, PD	J. Biol. Chem.	1996
12198488	The structural basis for specificity in human ABO(H) blood group biosynthesis Marcus, SL, Seto, NO, Borisova, SN, Evans, SV, Patenaude, SI, Szpacenko, A, Palcic, MM	Nat. Struct. Biol.	2002
10092863	Donor substrate specificity of recombinant human blood group A, B and hybrid A/B glycosyltransferases expressed in Escherichia coli Evans, SV, Bundle, DR, Palcic, MM, Compston, CA, Seto, NO, Narang, SA	Eur. J. Biochem.	1999
17259183	Structural effects of naturally occurring human blood group B galactosyltransferase mutations adjacent to the DXD motif Hosseini-Maaf, B, Borisova, SN, Persson, M, Olsson, ML, Evans, SV, Letts, JA, Palcic, MM	J. Biol. Chem.	2007