Oxysterol binds to the UGT1A3 gene:NR1H2,3:RXR:NCOR complex

Stable Identifier
R-HSA-9029517
Type
Reaction [binding]
Species
Homo sapiens
Compartment
Synonyms
Oxysterol binds to the UGT1A3 gene:LXR:RXR:NCOR complex
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UDP Glucuronosyltransferase Family 1 Member A3 (UGT1A3) is an enzyme that catalyzes the C24-glucuronidation of the primary bile acid (chenodeoxycholic acid, CDCA) and secondary bile acid (lithocholic acid, LCA). In human hepatoma HepG2 cells, an NR1H2,3 (liver X receptor, LXR) agonist T0901317 induced UGT1A3 expression in a dose- and time-dependent manner (Verreault M et al. 2006). Endogenous NR1H2,3 (LXR) agonists were also investigated by measuring UGT1A3 expression in HepG2 cells treated with 22R-hydroxycholesterol or 24S-hydroxycholesterol. Although the strongest increase in UGT1A3 mRNA levels was reached in the presence of T0901317, 24S-hydroxycholesterol treatment produced a significant induction of UGT1A3 expression, whereas 22R-hydroxycholesterol displayed no significant effects. Furthermore, UGT1A3 gene induction occured through binding of the LXRalpha:RXRalpha heterodimer to a functional LXR response element (LXRE) which was identified in the proximal part of the UGT1A3 promoter gene by site-directed mutagenesis, electrophoretic mobility shift assays and chromatin immunoprecipitation experiments (Verreault M et al. 2006).

Literature References
PubMed ID Title Journal Year
16871576 The liver X-receptor alpha controls hepatic expression of the human bile acid-glucuronidating UGT1A3 enzyme in human cells and transgenic mice

Verreault, M, Senekeo-Effenberger, K, Trottier, J, Bonzo, JA, BĂ©langer, J, Kaeding, J, Staels, B, Caron, P, Tukey, RH, Barbier, O

Hepatology 2006
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