Reactome | C3PO hydrolyzes cleaved passenger strand

C3PO hydrolyzes cleaved passenger strand

Stable Identifier

R-HSA-9023909

Type

Reaction [transition]

Species

Homo sapiens

Compartment

cytosol

ReviewStatus

5/5

Locations in the PathwayBrowser

Expand all

General

SBML | | PDF

SVG | | PPTX | SBGN

C3PO hydrolyzes cleaved passenger strand

Click the image above or here to open this reaction in the Pathway Browser
The layout of this reaction may differ from that in the pathway view due to the constraints in pathway layout

C3PO appears to act as a nuclease that hydrolyzes the passenger strand after cleavage by AGO2. C3PO could also be part of a DICER1-independent pathway for loading AGO2. AGO2 of humans may contain either miRNAs or siRNAs.
The mechanism that selects which strand is retained as the guide RNA is not well understood in humans. Overhanging nucleotides and strength of base-pairing at each end of the input duplex are observed to influence strand selection.

Literature References

PubMed ID	Title	Journal	Year
15284456	Argonaute2 is the catalytic engine of mammalian RNAi Liu, J, Carmell, MA, Rivas, FV, Marsden, CG, Thomson, JM, Song, JJ, Hammond, SM, Joshua-Tor, L, Hannon, GJ	Science	2004
18842624	Fluorescence correlation spectroscopy and fluorescence cross-correlation spectroscopy reveal the cytoplasmic origination of loaded nuclear RISC in vivo in human cells Ohrt, T, Mütze, J, Staroske, W, Weinmann, L, Höck, J, Crell, K, Meister, G, Schwille, P	Nucleic Acids Res	2008
22106413	Dicer is dispensable for asymmetric RISC loading in mammals Betancur, JG, Tomari, Y	RNA	2012
17531811	RNA helicase A interacts with RISC in human cells and functions in RISC loading Robb, GB, Rana, TM	Mol Cell	2007
19966796	ATP-dependent human RISC assembly pathways Yoda, M, Kawamata, T, Paroo, Z, Ye, X, Iwasaki, S, Liu, Q, Tomari, Y	Nat Struct Mol Biol	2010
16271386	Passenger-strand cleavage facilitates assembly of siRNA into Ago2-containing RNAi enzyme complexes Matranga, C, Tomari, Y, Shin, C, Bartel, DP, Zamore, PD	Cell	2005
21552258	Structure of C3PO and mechanism of human RISC activation Ye, X, Huang, N, Liu, Y, Paroo, Z, Huerta, C, Li, P, Chen, S, Liu, Q, Zhang, H	Nat. Struct. Mol. Biol.	2011
18782830	Effect of asymmetric terminal structures of short RNA duplexes on the RNA interference activity and strand selection Sano, M, Sierant, M, Miyagishi, M, Nakanishi, M, Takagi, Y, Sutou, S	Nucleic Acids Res	2008
17932509	Proteomic and functional analysis of Argonaute-containing mRNA-protein complexes in human cells Höck, J, Weinmann, L, Ender, C, Rüdel, S, Kremmer, E, Raabe, M, Urlaub, H, Meister, G	EMBO Rep	2007
19401777	Both strands of siRNA have potential to guide posttranscriptional gene silencing in mammalian cells Wei, JX, Yang, J, Sun, JF, Jia, LT, Zhang, Y, Zhang, HZ, Li, X, Meng, YL, Yao, LB, Yang, AG	PLoS One	2009