Reactome | RHOBTB1 binds interacting proteins at the endosome membrane

RHOBTB1 binds interacting proteins at the endosome membrane

Stable Identifier

R-HSA-9018778

Type

Reaction [binding]

Species

Homo sapiens

Compartment

plasma membrane, cytosol

ReviewStatus

5/5

Locations in the PathwayBrowser

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Signal Transduction (Homo sapiens)

- Signaling by Rho GTPases, Miro GTPases and RHOBTB3 (Homo sapiens)
  - Signaling by Rho GTPases (Homo sapiens)
    - RHO GTPase cycle (Homo sapiens)
      - RHOBTB GTPase Cycle (Homo sapiens)
        
        RHOBTB1 GTPase cycle (Homo sapiens)
        
        RHOBTB1 binds interacting proteins at the endosome membrane (Homo sapiens)

General

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RHOBTB1 binds interacting proteins at the endosome membrane

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RHOBTB1, like the other member of the RHOBTB family, RHOBTB2, as well as the divergent RHOBTB3, which does not belong to the RHO superfamily, binds to CUL3 (cullin-3), a core component of the BTB-CUL3-RBX2 E3 ubiquitin ligase complex (Berthold et al. 2008). RHOBTB1 binds to ROCK1 and ROCK2, and ROCK-mediated phosphorylation of RHOBTB1 may regulate its association with CUL3 (Haga et al. 2019). RHOBTB1 has been proposed as a substrate adaptor for PDE5, targeting it to CUL3 complexes for ubiquitination (Mukohda et al. 2019).

Candidate RHOBTB1 interactors identified in the screen conducted by Bagci et al. 2020 include:
CCT2 (Bagci et al. 2020)
CCT7 (Bagci et al. 2020)
COPS2 (Bagci et al. 2020)
COPS4 (Bagci et al. 2020)
CPSF7 (Bagci et al. 2020)
DBN1 (Bagci et al. 2020)
GPS1 (Bagci et al. 2020)
HNRNPC (Bagci et al. 2020)
MYO6 (Bagci et al. 2020)
RBBP6 (Bagci et al. 2020)
RBMX (Bagci et al. 2020)
RNF20 (Bagci et al. 2020)
SPEN (Bagci et al. 2020)
SRRM1 (Bagci et al. 2020)
STK38 (Bagci et al. 2020)
TRA2B (Bagci et al. 2020)
TXNL1 (Bagci et al. 2020)
VIM (Bagci et al. 2020)

Some of the candidate interactors, like CCT2 and CCT7, are part of the CCT chaperone complex, while others (DBN1 and MYO6) are related to the actin cytoskeleton, RNA splicing and export (HNRNPC, RBMX and SRRM1) or COP9 signalosome (COPS2 and COPS4).

Literature References

PubMed ID	Title	Journal	Year
31431478	RhoBTB1 interacts with ROCKs and inhibits invasion Ridley, AJ, Collu, F, Fraternali, F, Haga, RB, Borda D'Agua, B, Colomba, A, Garg, R, Menéndez, ST	Biochem. J.	2019
31871319	Mapping the proximity interaction network of the Rho-family GTPases reveals signalling pathways and regulatory mechanisms Tran, V, Gingras, AC, Elkholi, IE, Robert, A, Boulais, J, Faubert, D, Dubé, N, Hipfner, DR, Cote, JF, Lin, ZY, Bagci, H, Sriskandarajah, N, Thibault, MP	Nat. Cell Biol.	2020
30896450	RhoBTB1 protects against hypertension and arterial stiffness by restraining phosphodiesterase 5 activity Sigmund, CD, Nair, AR, Keen, HL, Guo, DF, Ibeawuchi, SC, Mukohda, M, Wu, J, Fang, S, Quelle, FW, Agbor, LN, Hu, C, Davis, DR, Lu, KT, Liu, X	J Clin Invest	2019
18835386	Characterization of RhoBTB-dependent Cul3 ubiquitin ligase complexes--evidence for an autoregulatory mechanism Furukawa, M, Miura, Y, Schenkova, K, Berthold, J, Ramos, S, Rivero, F, Aspenström, P	Exp. Cell Res.	2008