RHOBTB1 binds interacting proteins at the endosome membrane

Stable Identifier
Reaction [binding]
Homo sapiens
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RHOBTB1, like the other member of the RHOBTB family, RHOBTB2, as well as the divergent RHOBTB3, which does not belong to the RHO superfamily, binds to CUL3 (cullin-3), a core component of the BTB-CUL3-RBX2 E3 ubiquitin ligase complex (Berthold et al. 2008). RHOBTB1 binds to ROCK1 and ROCK2, and ROCK-mediated phosphorylation of RHOBTB1 may regulate its association with CUL3 (Haga et al. 2019). RHOBTB1 has been proposed as a substrate adaptor for PDE5, targeting it to CUL3 complexes for ubiquitination (Mukohda et al. 2019).

Candidate RHOBTB1 interactors identified in the screen conducted by Bagci et al. 2020 include:
CCT2 (Bagci et al. 2020)
CCT7 (Bagci et al. 2020)
COPS2 (Bagci et al. 2020)
COPS4 (Bagci et al. 2020)
CPSF7 (Bagci et al. 2020)
DBN1 (Bagci et al. 2020)
GPS1 (Bagci et al. 2020)
HNRNPC (Bagci et al. 2020)
MYO6 (Bagci et al. 2020)
RBBP6 (Bagci et al. 2020)
RBMX (Bagci et al. 2020)
RNF20 (Bagci et al. 2020)
SPEN (Bagci et al. 2020)
SRRM1 (Bagci et al. 2020)
STK38 (Bagci et al. 2020)
TRA2B (Bagci et al. 2020)
TXNL1 (Bagci et al. 2020)
VIM (Bagci et al. 2020)

Some of the candidate interactors, like CCT2 and CCT7, are part of the CCT chaperone complex, while others (DBN1 and MYO6) are related to the actin cytoskeleton, RNA splicing and export (HNRNPC, RBMX and SRRM1) or COP9 signalosome (COPS2 and COPS4).

Literature References
PubMed ID Title Journal Year
18835386 Characterization of RhoBTB-dependent Cul3 ubiquitin ligase complexes--evidence for an autoregulatory mechanism

Furukawa, M, Miura, Y, Schenkova, K, Berthold, J, Ramos, S, Rivero, F, Aspenström, P

Exp. Cell Res. 2008
31871319 Mapping the proximity interaction network of the Rho-family GTPases reveals signalling pathways and regulatory mechanisms

Tran, V, Gingras, AC, Elkholi, IE, Robert, A, Boulais, J, Faubert, D, Dubé, N, Hipfner, DR, Cote, JF, Lin, ZY, Bagci, H, Sriskandarajah, N, Thibault, MP

Nat. Cell Biol. 2020
30896450 RhoBTB1 protects against hypertension and arterial stiffness by restraining phosphodiesterase 5 activity

Sigmund, CD, Nair, AR, Keen, HL, Guo, DF, Ibeawuchi, SC, Mukohda, M, Wu, J, Fang, S, Quelle, FW, Agbor, LN, Hu, C, Davis, DR, Lu, KT, Liu, X

J Clin Invest 2019
31431478 RhoBTB1 interacts with ROCKs and inhibits invasion

Ridley, AJ, Collu, F, Fraternali, F, Haga, RB, Borda D'Agua, B, Colomba, A, Garg, R, Menéndez, ST

Biochem. J. 2019
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