Based on studies in mice, the intracellular domain of NOTCH3, NICD3, binds to transforming acidic coiled-coil protein-3 (TACC3). The interaction involves the ankyrin repeats of NOTCH3. The two proteins co-localize in the cytosol and possibly in the nucleus. TACC3 is implicated as a negative regulator of NOTCH signaling and may compete with NOTCH binding to RPBJ (Bargo et al. 2010).