Based on studies in mice, ROBO3.2 mRNA, which contains a premature stop codon, is recognized by components of the nonsense mediated decay (NMD) machinery during translation. Association of ROBO3.2 mRNA with UPF2 and UPF1 was directly demonstrated in mouse cells, and presence of other translation and NMD components is assumed (Colak et al. 2013). In this step, we only show association of ROBO3.2 mRNA with the UPF2-containing exon junction complex. For detailed representation of UPF2 and UPF1 in NMD, please refer to the Reactome pathway 'Nonsense Mediated Decay (NMD)'.