Reactome | RAC2 GAPs stimulate RAC2 GTPase activity

RAC2 GAPs stimulate RAC2 GTPase activity

Stable Identifier

R-HSA-9014295

Type

Reaction [transition]

Species

Homo sapiens

Compartment

cytosol, plasma membrane

ReviewStatus

5/5

Locations in the PathwayBrowser

Expand all

General

SBML | | PDF

SVG | | PPTX | SBGN

RAC2 GAPs stimulate RAC2 GTPase activity

Click the image above or here to open this reaction in the Pathway Browser
The layout of this reaction may differ from that in the pathway view due to the constraints in pathway layout

The following GTPase -activating proteins (GAPs) were shown to bind RAC2 and stimulate its GTPase activity, resulting in GTP to GDP hydrolysis and conversion of the active RAC2:GTP complex into the inactive RAC2:GDP complex (the high throughput study by Bagci et al. 2020 is cited as supporting evidence, because it examined binding of GAPs to constitutively active RAC2 mutant but did not examine GAP-mediated activation of RAC2 GTPase activity):
ARHGAP1 (Amin et al. 2016; supported by Bagci et al. 2020)
ARHGAP26 (Amin et al. 2016)
ARHGAP35 (Amin et al. 2016; Bagci et al. 2020)

The following GAPs, annotated as candidate RAC2 GAPs, were shown to bind RAC2 and stimulate its GTPase activity in some but not all studies or were shown to bind to constitutively active RAC2 mutant (Bagci et al. 2020) but their ability to activate RAC2 GTPase activity has not been tested:
ABR (Chuang et al. 1995: RAC2 directed GAP activity; Amin et al. 2016: no RAC2 directed GAP activity; Bagci et al. 2020: no binding t active RAC2)
ARHGAP17 (Amin et al. 2016: RAC2 directed GAP activity; Bagci et al. 2020: no binding to active RAC2)
ARHGAP21 (Bagci et al. 2020: binding to active RAC2)
ARHGAP32 (Bagci et al. 2020: binding to active RAC2)
ARHGAP39 (Bagci et al. 2020: binding to active RAC2)
ARHGAP42 (Bagci et al. 2020: binding to active RAC2)
BCR (Chuang et al. 1995: RAC2 directed GAP activity; Bagci et al. 2020: no binding to active RAC2)
DEPDC1B (Bagci et al. 2020)
OPHN1 (Amin et al. 2016: RAC2 directed GAP activity; Bagci et al. 2020: no binding to active RAC2)
PIK3R1 (Bagci et al. 2020: binding to active RAC2)
PIK3R2 (Bagci et al. 2020: binding to active RAC2)
RACGAP1 (Amin et al. 2016: RAC2 directed GAP activity; Bagci et al. 2020: no binding to active RAC2)
SYDE1 (Bagci et al. 2020: binding to active RAC2)

The following GAPs do not act on RAC2 or were shown by Bagci et al. 2020 to not bind to active RAC2:
ARAP2 (Bagci et al. 2020)
ARAP3 (Bagci et al. 2020)
ARHGAP5 (Bagci et al. 2020)
ARHGAP12 (Bagci et al. 2020)
ARHGAP29 (Bagci et al. 2020)
ARHGAP31 (Bagci et al. 2020)
DLC1 (Amin et al. 2016)
MYO9A (Bagci et al. 2020)
MYO9B (Bagci et al. 2020)
OCRL (Lichter Konecki et al. 2006; Erdmann et al. 2007; Bagci et al. 2020)
SRGAP2 (Bagci et al. 2020)
STARD8 (Amin et al. 2016)
STARD13 (Amin et al. 2016)

Literature References

PubMed ID	Title	Journal	Year
27481945	Deciphering the Molecular and Functional Basis of RHOGAP Family Proteins: A SYSTEMATIC APPROACH TOWARD SELECTIVE INACTIVATION OF RHO FAMILY PROTEINS Amin, E, Jaiswal, M, Derewenda, U, Reis, K, Nouri, K, Koessmeier, KT, Aspenström, P, Somlyo, AV, Dvorsky, R, Ahmadian, MR	J. Biol. Chem.	2016
17765681	A role of the Lowe syndrome protein OCRL in early steps of the endocytic pathway Erdmann, KS, Mao, Y, McCrea, HJ, Zoncu, R, Lee, S, Paradise, S, Modregger, J, Biemesderfer, D, Toomre, D, De Camilli, P	Dev. Cell	2007
16777452	The effect of missense mutations in the RhoGAP-homology domain on ocrl1 function Lichter-Konecki, U, Farber, LW, Cronin, JS, Suchy, SF, Nussbaum, RL	Mol. Genet. Metab.	2006
7479768	Abr and Bcr are multifunctional regulators of the Rho GTP-binding protein family Chuang, TH, Xu, X, Kaartinen, V, Heisterkamp, N, Groffen, J, Bokoch, GM	Proc. Natl. Acad. Sci. U.S.A.	1995
31871319	Mapping the proximity interaction network of the Rho-family GTPases reveals signalling pathways and regulatory mechanisms Bagci, H, Sriskandarajah, N, Robert, A, Boulais, J, Elkholi, IE, Tran, V, Lin, ZY, Thibault, MP, Dubé, N, Faubert, D, Hipfner, DR, Gingras, AC, Cote, JF	Nat. Cell Biol.	2020