RHOC GAPs stimulate RHOC GTPase activity

Stable Identifier
R-HSA-9013111
Type
Reaction [transition]
Species
Homo sapiens
Compartment
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The following GTPase activating proteins (GAPs) were shown to bind RHOC and stimulate its GTPase activity, resulting in GTP to GDP hydrolysis and conversion of the active RHOC:GTP complex into the inactive RHOC:GDP complex, or they were shown to bind to the constitutively active RHOC mutant in the high throughput screen conducted by Bagci et al. 2020, without testing for activation of RHOC GTPase activity (as indicated):
ARHGAP1 (Amin et al. 2016; supported by Bagci et al. 2020)
ARHGAP5 (Kusama et al. 2006; supported by Bagci et al. 2020)
ARHGAP18 (Chang et al. 2014)
ARHGAP26 (Amin et al. 2016)
DLC1 (Healy et al. 2008; Amin et al. 2016)
MYO9B (Graf et al. 2000; supported by Bagci et al. 2020)
RACGAP1 (Amin et al. 2016; supported by Bagci et al. 2020)
STARD13 (Amin et al. 2016)

The following GAPs were shown to bind RHOC and stimulate its GTPase activity in some but not all studies, or only their binding to the constitutively active RHOC mutant was shown without testing for activation of RHOC GTPase activity, and they are annotated as candidate RHOC GAPs:
ABR (Amin et al. 2016: RHOC directed GAP activity; Bagci et al. 2020: no binding to active RHOC)
ARHGAP21 (Lazarini et al. 2013: RHOC directed GAP activity; Bagci et al. 2020: no binding to active RHOC)
ARHGAP32 (Bagci et al. 2020: binding to active RHOC)
ARHGAP35 (Amin et al. 2016: RHOC directed GAP activity; Bagci et al. 2020: no binding to active RHOC)
ARHGAP39 (Bagci et al. 2020: binding to active RHOC)
BCR (Bagci et al. 2020: binding to active RHOC)
DEPDC1B (Bagci et al. 2020: binding to active RHOC)
OPHN1 (Amin et al. 2016: RHOC directed GAP activity; Bagci et al. 2020: no binding to active RHOC)
PIK3R1 (Bagci et al. 2020: binding to active RHOC)

The following GAPs do not act on RHOC or do not bind to the constitutively active RHOC mutant:
ARAP2 (Yoon et al. 2006; Bagci et al. 2020: no binding to active RHOC)
ARAP3 (Bagci et al. 2020: no binding to active RHOC)
ARHGAP12 (Bagci et al. 2020: no binding to active RHOC)
ARHGAP17 (Amin et al. 2016; Bagci et al. 2020: no binding to active RHOC)
ARHGAP29 (Bagci et al. 2020: no binding to active RHOC)
ARHGAP31 (Bagci et al. 2020: no binding to active RHOC)
ARHGAP42 (Bagci et al. 2020: no binding to active RHOC)
MYO9A (Bagci et al. 2020: no binding to active RHOC)
OCRL (Erdmann et al. 2006;Lichter Konecki et al. 2006; Bagci et al. 2020: no binding to active RHOC)
PIK3R2 (Bagci et al. 2020: no binding to active RHOC)
SRGAP2 (Bagci et al. 2020: no binding to active RHOC)
STARD8 (Amin et al. 2016)
SYDE1 (Bagci et al. 2020: no binding to active RHOC)

Literature References
PubMed ID Title Journal Year
15107133 Scambio, a novel guanine nucleotide exchange factor for Rho

Curtis, C, Hemmeryckx, B, Haataja, L, Senadheera, D, Groffen, J, Heisterkamp, N

Mol. Cancer 2004
17932950 DLC-1 suppresses non-small cell lung cancer growth and invasion by RhoGAP-dependent and independent mechanisms

Healy, KD, Hodgson, L, Kim, TY, Shutes, A, Maddileti, S, Juliano, RL, Hahn, KM, Harden, TK, Bang, YJ, Der, CJ

Mol. Carcinog. 2008
27481945 Deciphering the Molecular and Functional Basis of RHOGAP Family Proteins: A SYSTEMATIC APPROACH TOWARD SELECTIVE INACTIVATION OF RHO FAMILY PROTEINS

Amin, E, Jaiswal, M, Derewenda, U, Reis, K, Nouri, K, Koessmeier, KT, Aspenström, P, Somlyo, AV, Dvorsky, R, Ahmadian, MR

J. Biol. Chem. 2016
16896804 Gef10--the third member of a Rho-specific guanine nucleotide exchange factor subfamily with unusual protein architecture

Mohl, M, Winkler, S, Wieland, T, Lutz, S

Naunyn Schmiedebergs Arch. Pharmacol. 2006
16777452 The effect of missense mutations in the RhoGAP-homology domain on ocrl1 function

Lichter-Konecki, U, Farber, LW, Cronin, JS, Suchy, SF, Nussbaum, RL

Mol. Genet. Metab. 2006
23200924 ARHGAP21 is a RhoGAP for RhoA and RhoC with a role in proliferation and migration of prostate adenocarcinoma cells

Lazarini, M, Traina, F, Machado-Neto, JA, Barcellos, KS, Moreira, YB, Brandão, MM, Verjovski-Almeida, S, Ridley, AJ, Saad, ST

Biochim. Biophys. Acta 2013
7721814 Direct involvement of the small GTP-binding protein Rho in lbc oncogene function

Zheng, Y, Olson, MF, Hall, A, Cerione, RA, Toksoz, D

J. Biol. Chem. 1995
11207612 Functional role for the class IX myosin myr5 in epithelial cell infection by Shigella flexneri

Graf, B, Bähler, M, Hilpelä, P, Böwe, C, Adam, T

Cell. Microbiol. 2000
21454492 Mechanistic insights into specificity, activity, and regulatory elements of the regulator of G-protein signaling (RGS)-containing Rho-specific guanine nucleotide exchange factors (GEFs) p115, PDZ-RhoGEF (PRG), and leukemia-associated RhoGEF (LARG)

Jaiswal, M, Gremer, L, Dvorsky, R, Haeusler, LC, Cirstea, IC, Uhlenbrock, K, Ahmadian, MR

J. Biol. Chem. 2011
16776779 Inactivation of Rho GTPases by p190 RhoGAP reduces human pancreatic cancer cell invasion and metastasis

Kusama, T, Mukai, M, Endo, H, Ishikawa, O, Tatsuta, M, Nakamura, H, Inoue, M

Cancer Sci. 2006
17077126 ARAP2 effects on the actin cytoskeleton are dependent on Arf6-specific GTPase-activating-protein activity and binding to RhoA-GTP

Yoon, HY, Miura, K, Cuthbert, EJ, Davis, KK, Ahvazi, B, Casanova, JE, Randazzo, PA

J. Cell. Sci. 2006
25425145 ARHGAP18: an endogenous inhibitor of angiogenesis, limiting tip formation and stabilizing junctions

Chang, GH, Lay, AJ, Ting, KK, Zhao, Y, Coleman, PR, Powter, EE, Formaz-Preston, A, Jolly, CJ, Bower, NI, Hogan, BM, Rinkwitz, S, Becker, TS, Vadas, MA, Gamble, JR

Small GTPases 2014
19713215 Regulation of immature dendritic cell migration by RhoA guanine nucleotide exchange factor Arhgef5

Wang, Z, Kumamoto, Y, Wang, P, Gan, X, Lehmann, D, Smrcka, AV, Cohn, L, Iwasaki, A, Li, L, Wu, D

J. Biol. Chem. 2009
17765681 A role of the Lowe syndrome protein OCRL in early steps of the endocytic pathway

Erdmann, KS, Mao, Y, McCrea, HJ, Zoncu, R, Lee, S, Paradise, S, Modregger, J, Biemesderfer, D, Toomre, D, De Camilli, P

Dev. Cell 2007
22673745 Divergence of Rho residue 43 impacts GEF activity

Sloan, CM, Quinn, CV, Peters, JP, Farley, J, Goetzinger, C, Wernli, M, DeMali, KA, Ellerbroek, SM

Small GTPases 2012
23255595 Deciphering the molecular and functional basis of Dbl family proteins: a novel systematic approach toward classification of selective activation of the Rho family proteins

Jaiswal, M, Dvorsky, R, Ahmadian, MR

J. Biol. Chem. 2013
31871319 Mapping the proximity interaction network of the Rho-family GTPases reveals signalling pathways and regulatory mechanisms

Bagci, H, Sriskandarajah, N, Robert, A, Boulais, J, Elkholi, IE, Tran, V, Lin, ZY, Thibault, MP, Dubé, N, Faubert, D, Hipfner, DR, Gingras, AC, Cote, JF

Nat. Cell Biol. 2020
16112081 GrinchGEF--a novel Rho-specific guanine nucleotide exchange factor

Winkler, S, Mohl, M, Wieland, T, Lutz, S

Biochem. Biophys. Res. Commun. 2005
22911862 Identification of a novel actin-binding domain within the Rho guanine nucleotide exchange factor TEM4

Mitin, N, Rossman, KL, Der, CJ

PLoS ONE 2012
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GTPase activator activity of RHOC GAPs [cytosol]

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