RHOB GAPs stimulate RHOB GTPase activity

Stable Identifier
R-HSA-9013022
Type
Reaction [transition]
Species
Homo sapiens
Compartment
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The following GTPase activating proteins (GAPs) were shown to bind RHOB and stimulate its GTPase activity, resulting in GTP to GDP hydrolysis and conversion of the active RHOB:GTP complex into the inactive RHOB:GDP complex (the high throughput study by Bagci et al. 2020 examined binding to the active RHOB mutant but not activation of RHOB GTPase activity):
ABR (Amin et al. 2016, supported by Bagci et al. 2020)
ARHGAP1 (Amin et al. 2016, supported by Bagci et al. 2020)
ARHGAP5 (Kusama et al. 2006, supported by Bagci et al. 2020)
ARHGAP26 (Amin et al. 2016)
DLC1 (Healy et al. 2008, Amin et al. 2016)
MYO9B (Graf et al. 2000, supported by Bagci et al. 2020)
STARD13 (Amin et al. 2016)

The following GAPs were shown to bind RHOB and stimulate its GTPase activity in some but not all studies or have only been shown to bind to the active RHOB (as indicated) and are annotated as candidate RHOB GAPs:
ARHGAP21 (Bagci et al. 2020: binds to active RHOB)
ARHGAP32 (Bagci et al. 2020: binds to active RHOB)
ARHGAP35 (RHOB directed GAP activity: Amin et al. 2016; Bagci et al. 2020: no binding to active RHOB)
ARHGAP39 (Bagci et al. 2020: binds to active RHOB)
BCR (Bagci et al. 2020: binds to active RHOB)
DEPDC1B (Bagci et al. 2020: binds to active RHOB)
MYO9A (Bagci et al. 2020: binds to active RHOB)
OPHN1 (RHOB directed GAP activity: Amin et al. 2016; Bagci et al. 2020: no binding to active RHOB)
PIK3R1 (Bagci et al. 2020: binds to active RHOB)
PIK3R2 (Bagci et al. 2020: binds to active RHOB)
RACGAP1 (RHOB directed GAP activity: Amin et al. 2016; Bagci et al. 2020: no binding to active RHOB)
STARD8 (RHOB directed GAP activity: Braun et al. 2015; no RHOB directed GAP activity: Amin et al. 2016)

The following GAPs do not act on RHOB or were shown to not bind to the active RHOB, without testing for the ability to activate RHOB GTPase activity (as indicated):
ARAP2 (Bagci et al. 2020: does not bind active RHOB)
ARAP3 (Bagci et al. 2020: does not bind active RHOB)
ARHGAP12 (Bagci et al. 2020: does not bind active RHOB)
ARHGAP17 (Amin et al. 2016; Bagci et al. 2020: does not bind active RHOB)
ARHGAP29 (Bagci et al. 2020: does not bind active RHOB)
ARHGAP31 (Bagci et al. 2020: does not bind active RHOB)
ARHGAP42 (Bagci et al. 2020: does not bind active RHOB)
SRGAP2 (Bagci et al. 2020: does not bind active RHOB)
SYDE1 (Bagci et al. 2020: does not bind active RHOB)

Literature References
PubMed ID Title Journal Year
27481945 Deciphering the Molecular and Functional Basis of RHOGAP Family Proteins: A SYSTEMATIC APPROACH TOWARD SELECTIVE INACTIVATION OF RHO FAMILY PROTEINS

Amin, E, Jaiswal, M, Derewenda, U, Reis, K, Nouri, K, Koessmeier, KT, Aspenström, P, Somlyo, AV, Dvorsky, R, Ahmadian, MR

J. Biol. Chem. 2016
17932950 DLC-1 suppresses non-small cell lung cancer growth and invasion by RhoGAP-dependent and independent mechanisms

Healy, KD, Hodgson, L, Kim, TY, Shutes, A, Maddileti, S, Juliano, RL, Hahn, KM, Harden, TK, Bang, YJ, Der, CJ

Mol. Carcinog. 2008
11207612 Functional role for the class IX myosin myr5 in epithelial cell infection by Shigella flexneri

Graf, B, Bähler, M, Hilpelä, P, Böwe, C, Adam, T

Cell. Microbiol. 2000
31871319 Mapping the proximity interaction network of the Rho-family GTPases reveals signalling pathways and regulatory mechanisms

Bagci, H, Sriskandarajah, N, Robert, A, Boulais, J, Elkholi, IE, Tran, V, Lin, ZY, Thibault, MP, Dubé, N, Faubert, D, Hipfner, DR, Gingras, AC, Cote, JF

Nat. Cell Biol. 2020
16776779 Inactivation of Rho GTPases by p190 RhoGAP reduces human pancreatic cancer cell invasion and metastasis

Kusama, T, Mukai, M, Endo, H, Ishikawa, O, Tatsuta, M, Nakamura, H, Inoue, M

Cancer Sci. 2006
25673874 The Rho-specific GAP protein DLC3 coordinates endocytic membrane trafficking

Braun, AC, Hendrick, J, Eisler, SA, Schmid, S, Hausser, A, Olayioye, MA

J. Cell. Sci. 2015
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GTPase activator activity of RHOB GAPs [cytosol]

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