Reactome | RHOB GAPs stimulate RHOB GTPase activity

RHOB GAPs stimulate RHOB GTPase activity

Stable Identifier

R-HSA-9013022

Type

Reaction [transition]

Species

Homo sapiens

Compartment

cytosol, plasma membrane

ReviewStatus

5/5

Locations in the PathwayBrowser

Expand all

General

SBML | | PDF

SVG | | PPTX | SBGN

RHOB GAPs stimulate RHOB GTPase activity

Click the image above or here to open this reaction in the Pathway Browser
The layout of this reaction may differ from that in the pathway view due to the constraints in pathway layout

The following GTPase activating proteins (GAPs) were shown to bind RHOB and stimulate its GTPase activity, resulting in GTP to GDP hydrolysis and conversion of the active RHOB:GTP complex into the inactive RHOB:GDP complex (the high throughput study by Bagci et al. 2020 examined binding to the active RHOB mutant but not activation of RHOB GTPase activity):
ABR (Amin et al. 2016, supported by Bagci et al. 2020)
ARHGAP1 (Amin et al. 2016, supported by Bagci et al. 2020)
ARHGAP5 (Kusama et al. 2006, supported by Bagci et al. 2020)
ARHGAP26 (Amin et al. 2016)
DLC1 (Healy et al. 2008, Amin et al. 2016)
MYO9B (Graf et al. 2000, supported by Bagci et al. 2020)
STARD13 (Amin et al. 2016)

The following GAPs were shown to bind RHOB and stimulate its GTPase activity in some but not all studies or have only been shown to bind to the active RHOB (as indicated) and are annotated as candidate RHOB GAPs:
ARHGAP21 (Bagci et al. 2020: binds to active RHOB)
ARHGAP32 (Bagci et al. 2020: binds to active RHOB)
ARHGAP35 (RHOB directed GAP activity: Amin et al. 2016; Bagci et al. 2020: no binding to active RHOB)
ARHGAP39 (Bagci et al. 2020: binds to active RHOB)
BCR (Bagci et al. 2020: binds to active RHOB)
DEPDC1B (Bagci et al. 2020: binds to active RHOB)
MYO9A (Bagci et al. 2020: binds to active RHOB)
OPHN1 (RHOB directed GAP activity: Amin et al. 2016; Bagci et al. 2020: no binding to active RHOB)
PIK3R1 (Bagci et al. 2020: binds to active RHOB)
PIK3R2 (Bagci et al. 2020: binds to active RHOB)
RACGAP1 (RHOB directed GAP activity: Amin et al. 2016; Bagci et al. 2020: no binding to active RHOB)
STARD8 (RHOB directed GAP activity: Braun et al. 2015; no RHOB directed GAP activity: Amin et al. 2016)

The following GAPs do not act on RHOB or were shown to not bind to the active RHOB, without testing for the ability to activate RHOB GTPase activity (as indicated):
ARAP2 (Bagci et al. 2020: does not bind active RHOB)
ARAP3 (Bagci et al. 2020: does not bind active RHOB)
ARHGAP12 (Bagci et al. 2020: does not bind active RHOB)
ARHGAP17 (Amin et al. 2016; Bagci et al. 2020: does not bind active RHOB)
ARHGAP29 (Bagci et al. 2020: does not bind active RHOB)
ARHGAP31 (Bagci et al. 2020: does not bind active RHOB)
ARHGAP42 (Bagci et al. 2020: does not bind active RHOB)
SRGAP2 (Bagci et al. 2020: does not bind active RHOB)
SYDE1 (Bagci et al. 2020: does not bind active RHOB)

Literature References

PubMed ID	Title	Journal	Year
17932950	DLC-1 suppresses non-small cell lung cancer growth and invasion by RhoGAP-dependent and independent mechanisms Kim, TY, Shutes, A, Healy, KD, Juliano, RL, Hodgson, L, Harden, TK, Bang, YJ, Der, CJ, Maddileti, S, Hahn, KM	Mol. Carcinog.	2008
27481945	Deciphering the Molecular and Functional Basis of RHOGAP Family Proteins: A SYSTEMATIC APPROACH TOWARD SELECTIVE INACTIVATION OF RHO FAMILY PROTEINS Ahmadian, MR, Somlyo, AV, Amin, E, Koessmeier, KT, Jaiswal, M, Dvorsky, R, Reis, K, Derewenda, U, Nouri, K, Aspenström, P	J. Biol. Chem.	2016
11207612	Functional role for the class IX myosin myr5 in epithelial cell infection by Shigella flexneri Böwe, C, Bähler, M, Hilpelä, P, Adam, T, Graf, B	Cell. Microbiol.	2000
31871319	Mapping the proximity interaction network of the Rho-family GTPases reveals signalling pathways and regulatory mechanisms Tran, V, Gingras, AC, Elkholi, IE, Robert, A, Boulais, J, Faubert, D, Dubé, N, Hipfner, DR, Cote, JF, Lin, ZY, Bagci, H, Sriskandarajah, N, Thibault, MP	Nat. Cell Biol.	2020
16776779	Inactivation of Rho GTPases by p190 RhoGAP reduces human pancreatic cancer cell invasion and metastasis Endo, H, Nakamura, H, Tatsuta, M, Kusama, T, Inoue, M, Mukai, M, Ishikawa, O	Cancer Sci.	2006
25673874	The Rho-specific GAP protein DLC3 coordinates endocytic membrane trafficking Eisler, SA, Braun, AC, Hendrick, J, Hausser, A, Schmid, S, Olayioye, MA	J. Cell. Sci.	2015