FAHD1:Zn2+ dimer hydrolyses OA to PYR

Stable Identifier
Reaction [transition]
Homo sapiens
Locations in the PathwayBrowser
SVG |   | PPTX  | SBGN
Click the image above or here to open this reaction in the Pathway Browser
The layout of this reaction may differ from that in the pathway view due to the constraints in pathway layout

Fumarylacetoacetate hydrolase domain containing protein 1 (FAHD1) (Pircher et al. 2011, 2015, Jansen-Duerr et al. 2016) was identified to display a bi-functional catalytic mechanism (Weiss et al. 2018), being able to hydrolyse acylpyruvates (Pircher et al. 2011) similar to fumarylpyruvate hydrolase NagK of Ralstonia sp. (acetylpyruvate: vmax = 0,135 µmol/min/mg, KM = 4,6 µM), and to cleave oxaloacetate (OAA) via decarboxylation (Pircher et al. 2015) (OAA: vmax = 0,21 µmol/min/mg, KM = 32 µM). The enzyme is of dimeric form (Manjasetty et al. 2004) and uses Mg2+ or Mn2+ as cofactor. It is localized in the mitochondrial matrix (Pircher et al. 2011, Trukhina et al. 2002, Di Berardino et al. 1996). Its identification as ODx (Pircher et al. 2015) renders FAHD1 a possible antagonist to pyruvate carboxylase (PC) at a central position in the TCA cycle(Jansen-Duerr et al. 2016). It is believed that the ability of FAHD1 to decarboxylate OAA provides the basis of its requirement for maintaining healthy mitochondria in certain cells and tissues (Taferner et al. 2015, Petit et al. 2017). However, further studies of this topic are warranted.

Literature References
PubMed ID Title Journal Year
15551868 X-ray structure of fumarylacetoacetate hydrolase family member Homo sapiens FLJ36880

Sievert, V, Manjasetty, BA, Behlke, J, Götz, F, Büssow, K, Delbrück, H, Heinemann, U, Niesen, FH

Biol. Chem. 2004
25575590 Identification of FAH domain-containing protein 1 (FAHD1) as oxaloacetate decarboxylase

Liedl, KR, Kramer, C, Albertini, E, Pircher, H, Unterluggauer, H, Jansen-Dürr, P, von Grafenstein, S, Taferner, A, Metzger, C, Diener, T

J. Biol. Chem. 2015
Catalyst Activity

oxaloacetate decarboxylase activity of FAHD1:Mg2+ dimer [mitochondrial matrix]

Orthologous Events
Cross References
Cite Us!