Based on studies in mice, LHX2, a LIM-homeodomain transcription factor, directly represses transcription of the ROBO1 gene by binding to evolutionarily conserved LHX2 binding sites upstream of the ROBO1 gene promoter region. LHX2 is involved in thalamocortical axon guidance (Marcos-Mondejar et al. 2012). In commissural relay neurons of the dorsal spinal cord, however, ROBO1 expression is not affected by LHX2 (Wilson et al. 2008).
Transcription factors GBX2 and LMO3 may be indirectly involved in ROBO1 gene expression regulation by LHX2 (Chatterjee et al. 2012).