Based on studies in mice, TWIST1 and TWIST2 can bind, via their TWIST domain, to the Runt DNA binding domain of RUNX2. Binding to TWIST1 or TWIST2 inhibits transactivation activity of RUNX2 and delays the onset of osteoblast differentiation (Bialek et al. 2004). In addition, binding of RUNX2 to TWIST1 inhibits TWIST1-mediated induction of FGFR2 transcription (Lu et al. 2012). The interplay of RUNX2 and TWIST1 is implicated in the differentiation of odontoblasts (Li et al. 2011).