ROBO1, bound to SLIT2, binds to the RHO GAP protein MYO9B. The interaction involves all four cytoplasmic conserved (CC) motifs in the intracellular domain of ROBO1 and the RHO GAP domain of MYO9B. Binding to ROBO1 inhibits the RHOA GAP activity of MYO9B and increases the amount of active GTP-bound RHOA (RHOA:GTP). ROBO1-mediated inhibition of MYO9B and the resulting increase in RHOA activity is implicated as a negative regulator of invasiveness of lung cancer cells (Kong et al. 2015).