G-Protein Coupled Receptors (GPCR) sense extracellular signals and activate different Guanine nucleotide binding proteins (G-proteins) that have alpha, beta and gamma subunits. Upon activation, the alpha subunit of G-proteins dissociates from beta-gamma and the both are then free to regulate downstream effectors. Serine/threonine-protein kinase PAK 1 binds with Rho guanine nucleotide exchange factor 6 (ARHGEF6, PIX-Alpha) in the cytosol. PAK1 in this complex then binds to G-protein beta-gamma complex and facilitates the translocation of PAK1:ARHGEF6 complex to the plasma membrane, where Cell division control protein 42 homolog (CDC42) is activated. CDC42 is known to be involved in epithelial cell polarization processes.