NEDD8 and UBD bind NUB1 and the 26S proteasome

Stable Identifier
Reaction [binding]
Homo sapiens
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NEDD8 ultimate buster 1 (NUB1) is a negative regulator of the NEDD8 conjugation system. NUB1 interacts with NEDD8 and another ubiquitin-like modifier, UBD (also known as FAT10) to promote their degradation and that of their conjugated proteins (Kito et al, 2001; Kamitani et al, 2001; Hipp et al, 2004; Schmidtke et al, 2006). NUB1 interacts directly with both NEDD8/UBD and with PMSD4, a subunit of the 19S cap of the 26S proteasome, through a ubiquitin-like domain (UBL), and in this way promotes the contact between the proteasome and its substrate (Kito et al, 2001; Kamitani et al, 2001; Hipp et al, 2004; Tanji et al, 2005; Schmidtke et al, 2006; reviewed in Tanaka et al, 2012; Schmidtke et al, 2014). There are two isoforms of NUB1 in human cells that differ by the presence of a 14 amino acid insertion in the NUB1L. NUB1L promotes the degradation of NEDD8 more efficiently than the short isoform (Tanaka et al, 2003).

Literature References
PubMed ID Title Journal Year
11585840 Targeting of NEDD8 and its conjugates for proteasomal degradation by NUB1

Kito, K, Fukuda-Kamitani, T, Yeh, ET, Kamitani, T

J. Biol. Chem. 2001
11259415 NUB1, a NEDD8-interacting protein, is induced by interferon and down-regulates the NEDD8 expression

Kito, K, Kamitani, T, Yeh, ET

J. Biol. Chem. 2001
14757770 NEDD8 ultimate buster-1L interacts with the ubiquitin-like protein FAT10 and accelerates its degradation

Groettrup, M, Raasi, S, Hipp, MS, Schmidtke, G

J. Biol. Chem. 2004
12816948 Regulation of the NEDD8 conjugation system by a splicing variant, NUB1L

Yeh, ET, Tanaka, T, Kawashima, H, Kamitani, T

J. Biol. Chem. 2003
16171779 Interaction of NUB1 with the proteasome subunit S5a

Tanaka, T, Tanji, K, Kamitani, T

Biochem. Biophys. Res. Commun. 2005
16707496 The UBA domains of NUB1L are required for binding but not for accelerated degradation of the ubiquitin-like modifier FAT10

Groettrup, M, Hipp, MS, Schmidtke, G, Kalveram, B, Lukasiak, S, Bochtler, P, Weber, E

J. Biol. Chem. 2006
22306028 Inhibition of NEDD8-conjugation pathway by novel molecules: potential approaches to anticancer therapy

Tanaka, T, Nakatani, T, Kamitani, T

Mol Oncol 2012
23333871 FAT10ylation as a signal for proteasomal degradation

Aichem, A, Groettrup, M, Schmidtke, G

Biochim. Biophys. Acta 2014
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