AcM-UBE2M transfers NEDD8 to CUL9:RBX1

Stable Identifier
Reaction [transition]
Homo sapiens
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UBE2M transfers NEDD8 to lysine 1881 of CUL9 (Skaar et al, 2007; Li et al, 2014). Neddylation increases the ubiquitination activity of the E3 complex towards its targets (Hori et al, 1999; Duda et al, 2008). One defined target of CUL9 is BIRC5 (also known as Survivin), which has roles in cellular proliferation, inhibition of apoptosis and maintenance of genome stability (Zhao et al, 2000; Watanabe, 2010). CUL9-mediated ubiquitination of BIRC5 is negatively regulated by the 3M complex, consisting of CUL7, CCDC8 and OBSL1 (Li et al, 2014).
Literature References
PubMed ID Title Journal Year
17332328 PARC and CUL7 form atypical cullin RING ligase complexes

Tron, A, Florens, L, Tsutsumi, T, Skaar, JR, Swanson, SK, Arai, T, DeCaprio, JA, Washburn, MP

Cancer Res. 2007
10597293 Covalent modification of all members of human cullin family proteins by NEDD8

Kato, S, Shimbara, N, Miyamoto, C, Osaka, F, Okabayashi, K, Chiba, T, Tanaka, K, Hori, T

Oncogene 1999
24793696 CUL9 mediates the functions of the 3M complex and ubiquitylates survivin to maintain genome integrity

Pei, XH, Yan, J, Li, Z, Xiong, Y, Yan, F, Cappell, KM, Whitehurst, AW

Mol. Cell 2014
21447816 Temporal and spatial regulation of targeting aurora B to the inner centromere

Watanabe, Y

Cold Spring Harb. Symp. Quant. Biol. 2010
11069780 The ubiquitin-proteasome pathway regulates survivin degradation in a cell cycle-dependent manner

Downward, J, Lemoine, NR, Martins, LM, Tenev, T, Zhao, J

J. Cell. Sci. 2000
18805092 Structural insights into NEDD8 activation of cullin-RING ligases: conformational control of conjugation

Borg, LA, Hunt, HW, Duda, DM, Schulman, BA, Hammel, M, Scott, DC

Cell 2008
Catalyst Activity

NEDD8 transferase activity of NEDD8-AcM-UBE2M:CUL9:RBX1 [cytosol]

Orthologous Events
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