Reactome | PEX2:PEX10:PEX12 binds PEX5S,L (in PEX5S:PEX13:PEX14) and Ub:UBE2D1,2,3

PEX2:PEX10:PEX12 binds PEX5S,L (in PEX5S:PEX13:PEX14) and Ub:UBE2D1,2,3

Stable Identifier

R-HSA-8953917

Type

Reaction [binding]

Species

Homo sapiens

Compartment

peroxisomal membrane

ReviewStatus

5/5

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PEX2:PEX10:PEX12 binds PEX5S,L (in PEX5S:PEX13:PEX14) and Ub:UBE2D1,2,3

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A RING E3 ubiquitin ligase complex containing PEX10, PEX12, and PEX2 ubiquitinates PEX5L. The PEX2:PEX10:PEX12 complex is believed to bind an activated E2-ubiquitin conjugate (one of Ub:UBE2D1, Ub:UBE2D2, Ub:UBE2D3) and PEX5L in a complex that also contains PEX13 and PEX14 (Chang et al. 1999, Carvalho et al. 2007, Grou et al. 2008, Grou et al. 2009, Okumoto et al. 2011). The short isoform of PEX5, PEX5S, is inferred to undergo the same reaction.

Literature References

PubMed ID	Title	Journal	Year
10562279	PEX12 interacts with PEX5 and PEX10 and acts downstream of receptor docking in peroxisomal matrix protein import Gould, SJ, Chang, CC, Warren, DS, Sacksteder, KA	J. Cell Biol.	1999
18359941	Members of the E2D (UbcH5) family mediate the ubiquitination of the conserved cysteine of Pex5p, the peroxisomal import receptor Wiese, S, Sá-Miranda, C, Piechura, H, Grou, CP, Meyer, HE, Carvalho, AF, Warscheid, B, Azevedo, JE, Pinto, MP	J. Biol. Chem.	2008
19208625	Properties of the ubiquitin-pex5p thiol ester conjugate Huybrechts, SJ, Sá-Miranda, C, Grou, CP, Carvalho, AF, Azevedo, JE, Pinto, MP, Fransen, M	J. Biol. Chem.	2009
21554508	Cysteine ubiquitination of PTS1 receptor Pex5p regulates Pex5p recycling Miyata, N, Mukai, S, Okumoto, K, Misono, S, Matsumoto, Y, Fujiki, Y	Traffic	2011
17726030	Ubiquitination of mammalian Pex5p, the peroxisomal import receptor Sá-Miranda, C, Grou, CP, Carvalho, AF, Alencastre, IS, Azevedo, JE, Pinto, MP, Fransen, M	J. Biol. Chem.	2007