Reactome | AcM-UBE2M transfers NEDD8 to CRL1 E3 ubiquitin ligase complex

AcM-UBE2M transfers NEDD8 to CRL1 E3 ubiquitin ligase complex

Stable Identifier

R-HSA-8952618

Type

Reaction [transition]

Species

Homo sapiens

Compartment

cytosol

ReviewStatus

5/5

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AcM-UBE2M transfers NEDD8 to CRL1 E3 ubiquitin ligase complex

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UBE2M transfers NEDD8 to lysine 720 of CUL1 in the CRL E3 ubiquitin ligase complex (Hori et al, 1999; Duda et al, 2008). Neddylation increases the ubiquitination activity of the E3 complex towards its targets, and prevents binding of the CUL1 complex with the CAND1 inhibitor (Hori et al, 1999; Goldenberg et al, 2004; Duda et al, 2008; Kelsall et al, 2013; Scott et al, 2016). Targets of CUL1 RING complexes include a variety of cellular proteins including regulators of transcription and cell cycle progression, among others (reviewed in Lipkowitz and Weissman, 2011). CRL1 complexes are also hijacked by viruses, redirecting the ubiquitin ligase complex to target host proteins (reviewed in Mahon et al, 2014).

Literature References

PubMed ID	Title	Journal	Year
25314029	Cullin E3 ligases and their rewiring by viral factors Pick, E, Mahon, C, Krogan, NJ, Craik, CS	Biomolecules	2014
10597293	Covalent modification of all members of human cullin family proteins by NEDD8 Kato, S, Shimbara, N, Miyamoto, C, Osaka, F, Okabayashi, K, Chiba, T, Tanaka, K, Hori, T	Oncogene	1999
27565346	Two Distinct Types of E3 Ligases Work in Unison to Regulate Substrate Ubiquitylation Alpi, AF, Duda, DM, Paulo, JA, Schulman, BA, Ovaa, H, Rhee, DY, Harper, JW, Kelsall, IR, Olszewski, JL, Scott, DC, de Jong, A	Cell	2016
21863050	RINGs of good and evil: RING finger ubiquitin ligases at the crossroads of tumour suppression and oncogenesis Lipkowitz, S, Weissman, AM	Nat. Rev. Cancer	2011
24076655	TRIAD1 and HHARI bind to and are activated by distinct neddylated Cullin-RING ligase complexes Hofmann, K, Alpi, AF, Duda, DM, Schulman, BA, Knebel, A, Wood, N, Kelsall, IR, Wightman, M, Olszewski, JL, Langevin, F	EMBO J.	2013
15537541	Structure of the Cand1-Cul1-Roc1 complex reveals regulatory mechanisms for the assembly of the multisubunit cullin-dependent ubiquitin ligases Liu, J, Cascio, TC, Zheng, N, Xiong, Y, Shumway, SD, Garbutt, KC, Goldenberg, SJ	Cell	2004
18805092	Structural insights into NEDD8 activation of cullin-RING ligases: conformational control of conjugation Borg, LA, Hunt, HW, Duda, DM, Schulman, BA, Hammel, M, Scott, DC	Cell	2008