Reactome | AcM-UBE2F transfers NEDD8 to CRL5 E3 ubiquitin ligase complex

AcM-UBE2F transfers NEDD8 to CRL5 E3 ubiquitin ligase complex

Stable Identifier

R-HSA-8952044

Type

Reaction [transition]

Species

Homo sapiens

Compartment

cytosol

ReviewStatus

5/5

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AcM-UBE2F transfers NEDD8 to CRL5 E3 ubiquitin ligase complex

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UBE2F transfers NEDD8 to lysine 724 of CUL5 in the E3 ligase complex (Duda et al, 2008). Neddylation increases the ubiquitination activity of the E3 complex towards its target, and prevents binding of the CUL5 complex with the CAND1 inhibitor (Hori et al, 1999; Duda et al, 2008; Kelsall et al, 2013). Targets of CUL5 RING complexes include a variety of cellular proteins including receptor and non-receptor tyrosine kinases, signaling molecules transcriptional regulators (reviewed in Okamura et al, 2016). CRL5 complexes are also hijacked by viruses such as HIV, HPV and adenovirus among others. Interaction with viral proteins redirects the ubiquitin ligase complex to target host proteins to promote conditions that favor viral propagation (Harada et al, 2002; Mehle et al, 2004; reviewed in Mahon et al, 2014).

Literature References

PubMed ID	Title	Journal	Year
15574592	Phosphorylation of a novel SOCS-box regulates assembly of the HIV-1 Vif-Cul5 complex that promotes APOBEC3G degradation Santa-Marta, M, Goncalves, J, Gabuzda, D, McPike, M, Mehle, A	Genes Dev	2004
25314029	Cullin E3 ligases and their rewiring by viral factors Pick, E, Mahon, C, Krogan, NJ, Craik, CS	Biomolecules	2014
12186903	Analysis of the adenovirus E1B-55K-anchored proteome reveals its link to ubiquitination machinery Berk, AJ, Pallas, DC, Harada, JN, Shevchenko, A, Shevchenko, A	J. Virol.	2002
10597293	Covalent modification of all members of human cullin family proteins by NEDD8 Kato, S, Shimbara, N, Miyamoto, C, Osaka, F, Okabayashi, K, Chiba, T, Tanaka, K, Hori, T	Oncogene	1999
24076655	TRIAD1 and HHARI bind to and are activated by distinct neddylated Cullin-RING ligase complexes Hofmann, K, Alpi, AF, Duda, DM, Schulman, BA, Knebel, A, Wood, N, Kelsall, IR, Wightman, M, Olszewski, JL, Langevin, F	EMBO J.	2013
27030794	The role of cullin 5-containing ubiquitin ligases Okumura, F, Kamura, T, Joo-Okumura, A, Nakatsukasa, K	Cell Div	2016
18805092	Structural insights into NEDD8 activation of cullin-RING ligases: conformational control of conjugation Borg, LA, Hunt, HW, Duda, DM, Schulman, BA, Hammel, M, Scott, DC	Cell	2008