Transfer of NEDD8 to AcM-UBE2M

Stable Identifier
Reaction [transition]
Homo sapiens
Locations in the PathwayBrowser
SVG |   | PPTX  | SBGN
Click the image above or here to open this reaction in the Pathway Browser
The layout of this reaction may differ from that in the pathway view due to the constraints in pathway layout
UBA3:NAE1 transfers NEDD8 to the catalytic cysteine residue of UBE2M. Because two of the three E1-E2 interaction interfaces are created by conformational states present in the doubly-NEDDylated NAE1, transfer of NEDD8 to UBE2M is thought to weaken the interaction between UBA3:NAE1 and UBE2M, contributing to UBE2M release (Walden et al, 2003; Huang et al, 2004; Hunag et al, 2005; Huang et al, 2007).
Literature References
PubMed ID Title Journal Year
14690597 The structure of the APPBP1-UBA3-NEDD8-ATP complex reveals the basis for selective ubiquitin-like protein activation by an E1

Miller, DW, Huang, DT, Howard, RJ, Schulman, BA, Holton, JM, Minor, DL, Walden, H, Podgorski, MS

Mol. Cell 2003
17220875 Basis for a ubiquitin-like protein thioester switch toggling E1-E2 affinity

Huang, DT, Ohi, MD, Hunt, HW, Schulman, BA, Holton, JM, Zhuang, M

Nature 2007
15694336 Structural basis for recruitment of Ubc12 by an E2 binding domain in NEDD8's E1

Huang, DT, Schulman, BA, Holton, JM, Paydar, A, Waddell, MB, Zhuang, M

Mol. Cell 2005
15361859 A unique E1-E2 interaction required for optimal conjugation of the ubiquitin-like protein NEDD8

Miller, DW, Huang, DT, Cassell, R, Schulman, BA, Roussel, MF, Holton, JM, Mathew, R

Nat. Struct. Mol. Biol. 2004
Catalyst Activity

NEDD8 transferase activity of AcM-UBE2M:NEDD8-UBA3:NAE1:NEDD8 [cytosol]

Orthologous Events
Cite Us!