IL12B dimerizes

Stable Identifier
R-HSA-8950456
Type
Reaction [transition]
Species
Homo sapiens
Compartment
endoplasmic reticulum lumen
Synonyms
Interleukin-12 subunit beta binds Interleukin-12 subunit beta forming Interleukin-12 beta homodimer, IL-12B binds IL-12B to for a IL12B homodimer
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IL12B dimerizes
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Murine cells have been shown to produce Interleukin-12 homodimer (IL-12p80) (Gillessen et al. 1995). In a cell-free system porcine Interleukin 12 homodimer (IL-12p80) is formed from two subunits of Interleukin 12 subunit beta (IL12B, IL-12p40), stabilized by a disulfide link. The cysteine in position 197 of IL12B is required for homodimer formation. Prolyl 4-hydroxylase subunit beta (P4HB), in this article referred to as protein disulfide isomerase (PDI), can act as a chaperone to induce homodimer formation (Martens et al. 2000). Human cells do not produce IL-12p80 under normal conditions (Ling et al. 1995), when however expressed acts as an antagonist of Interleukin-12 signaling, competing with the Interleukin 12 heterodimer (IL12) for its receptor (Ling et al. 1995).

Literature References
PubMed ID Title Journal Year
25259790 Recombinant p35 from bacteria can form Interleukin (IL-)12, but Not IL-35

Aparicio-Siegmund, S, Moll, JM, Lokau, J, Grusdat, M, Schröder, J, Plöhn, S, Rose-John, S, Grötzinger, J, Lang, PA, Scheller, J, Garbers, C

PLoS ONE 2014
7527811 Human IL-12 p40 homodimer binds to the IL-12 receptor but does not mediate biologic activity

Ling, P, Gately, MK, Gubler, U, Stern, AS, Lin, P, Hollfelder, K, Su, C, Pan, YC, Hakimi, J

J. Immunol. 1995
11054122 Protein disulfide isomerase-mediated cell-free assembly of recombinant interleukin-12 p40 homodimers

Martens, E, Alloza, I, Scott, CJ, Billiau, A, Vandenbroeck, K

Eur. J. Biochem. 2000
Participants
Input
Output
Participant Of
hasEvent
Catalyst Activity
Catalyst Activity
Title
protein disulfide isomerase activity of P4HB [endoplasmic reticulum lumen]
Physical Entity
P4HB [endoplasmic reticulum lumen]
Activity
protein disulfide isomerase activity (0003756)
Orthologous Events
IL12B dimerizes (Bos taurus)
IL12B dimerizes (Caenorhabditis elegans)
IL12B dimerizes (Mus musculus)
IL12B dimerizes (Rattus norvegicus)
IL12B dimerizes (Sus scrofa)
IL12B dimerizes (Xenopus tropicalis)
Authored
Duenas, C  (2016-12-02)
Reviewed
van de Vosse, E  (2017-05-12)
Created
Duenas, C  (2016-12-02)