In response to a stalled replication fork, SHPRH polyubiquitinates lysine-164 of PCNA that has already been monoubiquitinated on lysine-164 by RAD18:UBE2B (RAD18:RAD6) (Unk et al. 2006, Motegi et al. 2006, Motegi et al. 2008). The ubiquitin donor is the E2 complex UBE2N:UBE2V2 (UBC13:MMS2) containing ubiquitin conjugated to UBE2N. The resulting polyubiquitin chain contains lysine-63 (K63) linkages and appears to change the repair process from translesion synthesis (TLS) to template switching (TS). SHPRH interacts directly with PCNA, RAD18:UBE2B, and UBE2N:UBE2V2.
Roest, HP, Ding, H, Motegi, A, Myung, K, Markowitz, SD, Wu, X, Liaw, HJ, Lee, KY, Moinova, H, Hoeijmakers, JH, Maas, A
Haracska, L, Hurwitz, J, Blastyák, A, Bermudez, V, Prakash, L, Fatyol, K, Hajdu, I, Unk, I, Szakál, B, Prakash, S
Motegi, A, Myung, K, Markowitz, SD, Sood, R, Liu, PP, Moinova, H
ubiquitin protein ligase activity of SHPRH:monoUb:K164-PCNA:RAD18:UBE2B:Ub:UBE2N:UBE2V2 [nucleoplasm]
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