RNF20:RNF40 monoubiquitinates Histone H2B

Stable Identifier
Reaction [omitted]
Homo sapiens
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The ubiquitin E3 ligase complex RNF20:RNF40 interacts with the PAF complex (Kim et al. 2009) that is associated with RNA polymerase II via WAC (Zhang and Yu 2011) at transcriptionally active genes (Zhe et al. 2005). RNF20:RNF40 monoubiquitinates nucleosomal histone H2B on lysine-120 (lysine-121 of the unprocessed histone H2B) using UBE2A,B:Ubiquitin as the ubiquitin donor (Zhu et al. 2005, Kim et al. 2009, Zhang and Yu 2011, Zhang et al. 2014, Dickson et al. 2016). Monoubiquitination of histone H2B leads to methylation of lysine-4 and lysine-79 of histone H3, marks of active chromatin (Zhu et al. 2005). Arsenite binds the RING domains of RNF20 and RNF40 and inhibits the ubiquitination of histone H2B (Zhang et al. 2014).
Literature References
PubMed ID Title Journal Year
19410543 RAD6-Mediated transcription-coupled H2B ubiquitylation directly stimulates H3K4 methylation in human cells

Kim, J, Shilatifard, A, Tang, Z, Roeder, RG, Guermah, M, Milne, TA, Lee, JS, Muir, TW, McGinty, RK

Cell 2009
16307923 Monoubiquitination of human histone H2B: the factors involved and their roles in HOX gene regulation

Pham, AD, Zheng, Y, Reinberg, D, Mandal, SS, Tempst, P, Erdjument-Bromage, H, Zhu, B

Mol. Cell 2005
27798111 The RING finger domain E3 ubiquitin ligases BRCA1 and the RNF20/RNF40 complex in global loss of the chromatin mark histone H2B monoubiquitination (H2Bub1) in cell line models and primary high-grade serous ovarian cancer

Chou, A, Cole, AJ, Clarkson, A, Kennedy, CJ, Dickson, KA, Gill, AJ, Henderson, BR, Gard, GB

Hum. Mol. Genet. 2016
25170678 Arsenite binds to the RING finger domains of RNF20-RNF40 histone E3 ubiquitin ligase and inhibits DNA double-strand break repair

Dai, X, Zhang, F, Wang, P, Cai, Q, Song, J, Wang, Y, Seidman, MM, Lin, K, Paramasivam, M

J. Am. Chem. Soc. 2014
21329877 WAC, a functional partner of RNF20/40, regulates histone H2B ubiquitination and gene transcription

Yu, X, Zhang, F

Mol. Cell 2011
Catalyst Activity

ubiquitin protein ligase activity of PAF1c:WAC:RNF20:RNF40:Ub:UBE2A,B:HIST1H2B [nucleoplasm]

Orthologous Events
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