NT5E:Zn2+ hydrolyses NAD+

Stable Identifier
R-HSA-8940070
Type
Reaction [transition]
Species
Homo sapiens
Compartment
extracellular region, plasma membrane
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NT5E:Zn2+ hydrolyses NAD+
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5'-nucleotidase (NT5E, CD73) is able to hydrolyse extracellular nucleotides into membrane permeable nucleosides. It displays a broad specificity, acting on mono- or di-nucleotide nicotinamides and different adenosine phosphates, with maximal activity on 5'-adenosine monophosphate. Human NT5E can hydrolyse both NAD+ and NMN, suggesting a role in NAD metabolism (Garavaglia et al. 2012). NT5E is a glycolipid-anchored plasma membrane enzyme (Misumi et al. 1990) that is active in dimeric form and requires one zinc ion per subunit (Zimmermann 1992).

Literature References
PubMed ID Title Journal Year
2129526 Primary structure of human placental 5'-nucleotidase and identification of the glycolipid anchor in the mature form

Misumi, Y, Ogata, S, Ohkubo, K, Hirose, S, Ikehara, Y

Eur J Biochem 1990
1637327 5'-nucleotidase: molecular structure and functional aspects

Zimmermann, H

Biochem J 1992
21933152 The high-resolution crystal structure of periplasmic Haemophilus influenzae NAD nucleotidase reveals a novel enzymatic function of human CD73 related to NAD metabolism

Garavaglia, S, Bruzzone, S, Cassani, C, Canella, L, Allegrone, G, Sturla, L, Mannino, E, Millo, E, De Flora, A, Rizzi, M

Biochem. J. 2012
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Catalyst Activity
Title
5'-nucleotidase activity of NT5E:Zn2+ dimer [plasma membrane]
Physical Entity
NT5E:Zn2+ dimer [plasma membrane]
Activity
5'-nucleotidase activity (0008253)
Orthologous Events
NT5E:Zn2+ hydrolyses NAD+ (Bos taurus)
NT5E:Zn2+ hydrolyses NAD+ (Canis familiaris)
NT5E:Zn2+ hydrolyses NAD+ (Danio rerio)
NT5E:Zn2+ hydrolyses NAD+ (Drosophila melanogaster)
NT5E:Zn2+ hydrolyses NAD+ (Gallus gallus)
NT5E:Zn2+ hydrolyses NAD+ (Mus musculus)
NT5E:Zn2+ hydrolyses NAD+ (Rattus norvegicus)
NT5E:Zn2+ hydrolyses NAD+ (Sus scrofa)
NT5E:Zn2+ hydrolyses NAD+ (Xenopus tropicalis)
Authored
Jassal, B  (2016-09-23)
Reviewed
D'Eustachio, P  (2016-10-11)
Created
Jassal, B  (2016-09-23)