Reactome | NT5E:Zn2+ hydrolyses NAD+

NT5E:Zn2+ hydrolyses NAD+

Stable Identifier

R-HSA-8940070

Type

Reaction [transition]

Species

Homo sapiens

Compartment

extracellular region, plasma membrane

ReviewStatus

5/5

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5'-nucleotidase (NT5E, CD73) is able to hydrolyse extracellular nucleotides into membrane permeable nucleosides. It displays a broad specificity, acting on mono- or di-nucleotide nicotinamides and different adenosine phosphates, with maximal activity on 5'-adenosine monophosphate. Human NT5E can hydrolyse both NAD+ and NMN, suggesting a role in NAD metabolism (Garavaglia et al. 2012). NT5E is a glycolipid-anchored plasma membrane enzyme (Misumi et al. 1990) that is active in dimeric form and requires one zinc ion per subunit (Zimmermann 1992).

Literature References

PubMed ID	Title	Journal	Year
1637327	5'-nucleotidase: molecular structure and functional aspects Zimmermann, H	Biochem J	1992
2129526	Primary structure of human placental 5'-nucleotidase and identification of the glycolipid anchor in the mature form Ogata, S, Ikehara, Y, Misumi, Y, Hirose, S, Ohkubo, K	Eur J Biochem	1990
21933152	The high-resolution crystal structure of periplasmic Haemophilus influenzae NAD nucleotidase reveals a novel enzymatic function of human CD73 related to NAD metabolism Sturla, L, Canella, L, Garavaglia, S, Mannino, E, Rizzi, M, Allegrone, G, Millo, E, Bruzzone, S, De Flora, A, Cassani, C	Biochem. J.	2012