RAB9 GEFs exchange GTP for GDP on RAB9

Stable Identifier
Reaction [transition]
Homo sapiens
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RAB9 plays a role in the retrograde traffic of cargo such as the mannose-6-phosphate receptors (M6PR) from the late endosome to the trans-Golgi network (TGN). Vesicles are recruited to the TGN through interaction of RAB9 with the atypical RHO GTPase RHOBTB3, and tethered by virtue of interaction with TGN-localized Golgins and the GARP complex (Perez-Victoria et al, 2008; Perez-Victoria et al, 2009; Diaz et al, 1999; Espinosa et al, 2009; reviewed in Pfeffer, 2011; Chia and Gleeson, 2014). Interaction of RAB9:GDP with its GEFs promotes release of GDP, allowing GTP to bind, and precludes the interaction of RAB9 with GDI and CHM proteins. DENND2 family members have been shown to be RAB9 specific GEFs, and HeLa cells depleted of RAB9 or DENND2A show reduced staining of M6PR and a loss of M6PR-positive structures in the cell periphery (Yoshimura et al, 2010).
Literature References
PubMed ID Title Journal Year
18367545 Requirement of the human GARP complex for mannose 6-phosphate-receptor-dependent sorting of cathepsin D to lysosomes

Mardones, GA, Pérez-Victoria, FJ, Bonifacino, JS

Mol. Biol. Cell 2008
20937701 Family-wide characterization of the DENN domain Rab GDP-GTP exchange factors

Linford, A, Rigden, DJ, Barr, FA, Gerondopoulos, A, Yoshimura, S

J. Cell Biol. 2010
25343031 Membrane tethering

Chia, PZ, Gleeson, PA

F1000Prime Rep 2014
19620288 Dual roles of the mammalian GARP complex in tethering and SNARE complex assembly at the trans-golgi network

Pérez-Victoria, FJ, Bonifacino, JS

Mol. Cell. Biol. 2009
25341920 Rab proteins and the compartmentalization of the endosomal system

Wandinger-Ness, A, Zerial, M

Cold Spring Harb Perspect Biol 2014
21421921 Entry at the trans-face of the Golgi

Pfeffer, SR

Cold Spring Harb Perspect Biol 2011
9590177 TIP47: a cargo selection device for mannose 6-phosphate receptor trafficking

Pfeffer, SR, Díaz, E

Cell 1998
27246931 Multiple Types of Guanine Nucleotide Exchange Factors (GEFs) for Rab Small GTPases

Oguchi, ME, Fukuda, M, Ishida, M

Cell Struct. Funct. 2016
19490898 RhoBTB3: a Rho GTPase-family ATPase required for endosome to Golgi transport

Espinosa, EJ, Calero, M, Sridevi, K, Pfeffer, SR

Cell 2009
Catalyst Activity

guanyl-nucleotide exchange factor activity of RAB9 GEFs [cytosol]

Orthologous Events
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