DENND4s exchange GTP for GDP on RAB10

Stable Identifier
Reaction [transition]
Homo sapiens
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RAB10 has well characterized roles in basolateral sorting and GLUT4 transport and has more recently been shown to contribute to ER dynamics and morphology (Chen et al, 1993; Babbey et al, 2006; Schuck et al, 2007; Sano et al, 2008; Chen et al, 2012; Chen and Lippincott-Schwarz, 2013; English and Voeltz, 2013). In vitro, DENN4 family members A, B and C show RAB10-specific GEF activity, and DENN4B staining colocalizes with that of RAB10 in HeLa cells (Yoshimura et al, 2010). Consistent with this, coexpression of DENND4C and RAB10 in HEK293 cells increases the fraction of GTP-bound RAB10, and DENND4C knockdown in insulin-stimulated 3T3 cells reduces the trafficking of GLUT4 to the plasma membrane (Sano et al, 2011). Interaction of RAB10:GDP with its GEFs promotes release of GDP, allowing GTP to bind, and precludes the interaction of RAB10 with GDI and CHM proteins.
Literature References
PubMed ID Title Journal Year
20937701 Family-wide characterization of the DENN domain Rab GDP-GTP exchange factors

Linford, A, Rigden, DJ, Barr, FA, Gerondopoulos, A, Yoshimura, S

J. Cell Biol. 2010
17132146 Rab10 is involved in basolateral transport in polarized Madin-Darby canine kidney cells

Ang, A, Schuck, S, Gerl, MJ, Keller, P, Manninen, A, Mellman, I, Simons, K

Traffic 2007
23263280 Rab10 GTPase regulates ER dynamics and morphology

Voeltz, GK, English, AR

Nat. Cell Biol. 2013
22908308 Rab10 and myosin-Va mediate insulin-stimulated GLUT4 storage vesicle translocation in adipocytes

Lippincott-Schwartz, J, Zhang, J, Chen, Y, Jiang, L, Song, E, Deng, Y, Xu, T, Hammer, JA, Wu, XS, Wang, Y

J. Cell Biol. 2012
25341920 Rab proteins and the compartmentalization of the endosomal system

Wandinger-Ness, A, Zerial, M

Cold Spring Harb Perspect Biol 2014
18076383 Rab10 in insulin-stimulated GLUT4 translocation

Sano, H, Fukuda, M, Peck, GR, Roach, WG, Lienhard, GE

Biochem. J. 2008
16641372 Rab10 regulates membrane transport through early endosomes of polarized Madin-Darby canine kidney cells

Grant, BD, Wang, E, Dunn, KW, Ahktar, N, Babbey, CM, Chen, CC

Mol. Biol. Cell 2006
27246931 Multiple Types of Guanine Nucleotide Exchange Factors (GEFs) for Rab Small GTPases

Oguchi, ME, Fukuda, M, Ishida, M

Cell Struct. Funct. 2016
7688123 Expression and localization of two low molecular weight GTP-binding proteins, Rab8 and Rab10, by epitope tag

Chen, YT, Holcomb, C, Moore, HP

Proc. Natl. Acad. Sci. U.S.A. 1993
23713133 Rab10 delivers GLUT4 storage vesicles to the plasma membrane

Lippincott-Schwartz, J, Chen, Y

Commun Integr Biol 2013
Catalyst Activity

guanyl-nucleotide exchange factor activity of DENND4s [cytosol]

Orthologous Events
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