RAB5 GEFs exchange GTP for GDP on RAB5

Stable Identifier
Reaction [transition]
Homo sapiens
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RAB5 has roles at early endosomal compartments including fusion of vesicles originating from the plasma membrane with early endosomes, and is implicated in clathrin-mediated endocytosis (Chavrier et al, 1990; Bucci et al, 1992; Gorvel et al, 1991; Hoffenberg et al, 2000; Zeigerer et al, 2012; Taylor et al, 2011). RAB5 activation is mediated by a number of GEFs including members of the RIN (RAS and RAB interactor) and ALS2 families and GAPVD1, all of which belong to the VPS9-domain containing group (Horiuchi et al, 1997; Tall et al, 2001; Saito et al, 2002; Kajiho et al, 2003; Kajiho et al, 2011; Kunita et al, 2004; Hadano et al, 2004; Hunker et al, 2006; Suzuki-Utsunomiya et al, 2007; reviewed in Ishida et al, 2016). Interaction of RAB5 with its GEFs promotes release of GDP, allowing GTP to bind, and precludes the interaction of RAB5 with GDI and CHM proteins.

Literature References
PubMed ID Title Journal Year
9323142 A novel Rab5 GDP/GTP exchange factor complexed to Rabaptin-5 links nucleotide exchange to effector recruitment and function

Woodman, P, Ashman, K, Wilm, M, Horiuchi, H, McBride, HM, Rybin, V, Zerial, M, Rubino, M, Lippé, R, Mann, M, Stenmark, H

Cell 1997
12972505 RIN3: a novel Rab5 GEF interacting with amphiphysin II involved in the early endocytic pathway

Tsujita, K, Araki, Y, Saito, K, Kajiho, H, Kurosu, H, Katada, T, Kontani, K

J. Cell. Sci. 2003
12427552 Molecular cloning and characterization of prokineticin receptors

Ohishi, T, Soga, T, Kamohara, M, Hiyama, H, Matsumoto, S, Furuichi, K, Oda, T, Matsushime, H, Takasaki, J, Saito, T

Biochim Biophys Acta 2002
10818110 A novel membrane-anchored Rab5 interacting protein required for homotypic endosome fusion

Aballay, A, Dickey, BF, Knoll, BJ, Hall, HS, Barbieri, MA, Liu, X, Stahl, PD, Baughn, RE, Hoffenberg, S, Dai, W, Nikolova, L

J. Biol. Chem. 2000
2115402 Localization of low molecular weight GTP binding proteins to exocytic and endocytic compartments

Parton, RG, Hauri, HP, Zerial, M, Chavrier, P, Simons, K

Cell 1990
21586568 Characterization of RIN3 as a guanine nucleotide exchange factor for the Rab5 subfamily GTPase Rab31

Nakagawa, S, Kajiho, H, Minoda, T, Katada, T, Yoshikawa, M, Fukushima, S, Kontani, K, Sakurai, K

J. Biol. Chem. 2011
1900457 rab5 controls early endosome fusion in vitro

Chavrier, P, Gruenberg, J, Zerial, M, Gorvel, JP

Cell 1991
17239822 ALS2CL, a novel ALS2-interactor, modulates ALS2-mediated endosome dynamics

Kunita, R, Suzuki-Utsunomiya, K, Mizumura, H, Osuga, H, Hadano, S, Otomo, A, Ikeda, JE

Biochem. Biophys. Res. Commun. 2007
1516130 The small GTPase rab5 functions as a regulatory factor in the early endocytic pathway

Mather, IH, Hoflack, B, Bucci, C, Parton, RG, Zerial, M, Simons, K, Stunnenberg, H

Cell 1992
15247254 Homo-oligomerization of ALS2 through its unique carboxyl-terminal regions is essential for the ALS2-associated Rab5 guanine nucleotide exchange activity and its regulatory function on endosome trafficking

Kunita, R, Mizumura, H, Suzuki, K, Showguchi-Miyata, J, Yanagisawa, Y, Hadano, S, Otomo, A, Ikeda, JE

J. Biol. Chem. 2004
11703925 Ras-activated endocytosis is mediated by the Rab5 guanine nucleotide exchange activity of RIN1

Horazdovsky, BF, Barbieri, MA, Stahl, PD, Tall, GG

Dev. Cell 2001
15388334 ALS2CL, the novel protein highly homologous to the carboxy-terminal half of ALS2, binds to Rab5 and modulates endosome dynamics

Kunita, R, Suzuki-Utsunomiya, K, Mizumura, H, Showguchi-Miyata, J, Yanagisawa, Y, Hadano, S, Otomo, A, Ikeda, JE

FEBS Lett. 2004
25341920 Rab proteins and the compartmentalization of the endosomal system

Wandinger-Ness, A, Zerial, M

Cold Spring Harb Perspect Biol 2014
21445324 A high precision survey of the molecular dynamics of mammalian clathrin-mediated endocytosis

Merrifield, CJ, Taylor, MJ, Perrais, D

PLoS Biol. 2011
16410077 Rab5-activating protein 6, a novel endosomal protein with a role in endocytosis

Barbieri, MA, Galvis, A, Kruk, I, Giambini, H, Hunker, CM, Veisaga, ML

Biochem. Biophys. Res. Commun. 2006
22622570 Rab5 is necessary for the biogenesis of the endolysosomal system in vivo

Koteliansky, V, Kalaidzidis, Y, Bogorad, RL, Epstein-Barash, H, Marsico, G, Del Conte-Zerial, P, Zerial, M, Peng, CG, Gilleron, J, Hengstler, JG, Kuchimanchi, S, Zeigerer, A, Nonaka, H, Seifert, S, Ruda, VM

Nature 2012
27246931 Multiple Types of Guanine Nucleotide Exchange Factors (GEFs) for Rab Small GTPases

Oguchi, ME, Fukuda, M, Ishida, M

Cell Struct. Funct. 2016
Catalyst Activity

guanyl-nucleotide exchange factor activity of RAB5 GEFs [cytosol]

Orthologous Events
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