Clathrin recruits auxilins to the clathrin-coated vesicle

Stable Identifier
Reaction [binding]
Homo sapiens
Locations in the PathwayBrowser
SVG |   | PPTX  | SBGN
Click the image above or here to open this reaction in the Pathway Browser
The layout of this reaction may differ from that in the pathway view due to the constraints in pathway layout

After fission from the plasma membrane, auxilin proteins DNAJC6 and GAK are recruited to the vesicle through interaction with clathrin and phosphoinositides, in particular PI4P (Greener et al, 2000; Lee et al, 2006; Massol et al, 2006; Taylor et al, 2011; Scheele et al, 2001; Fotin et al, 2004a; Fotin et al, 2004b; Guan et al, 2010; reviewed in McMahon and Boucrot, 2011; Sousa and Lafer, 2015). Auxilin in turn recruits the ATPase HSPA8 (also known as HSC70) , which uses the energy from ATP hydrolysis to remove the clathrin-coat from the vesicle, priming it for fusion with a subsequent endosomal compartment (Schlossman et al, 1984; Ungewickell et al, 1995; Rappoport et al, 2008; Xing et al, 2010; Bocking et al, 2011; Rothnie et al, 2011; reviewed in McMahon and Boucrot, 2011; Sousa and Lafer, 2015).

Literature References
PubMed ID Title Journal Year
21779028 Molecular mechanism and physiological functions of clathrin-mediated endocytosis

Boucrot, E, McMahon, HT

Nat. Rev. Mol. Cell Biol. 2011
10625686 Role of cyclin G-associated kinase in uncoating clathrin-coated vesicles from non-neuronal cells

Nojima, H, Zhao, X, Greener, T, Eisenberg, E, Greene, LE

J. Biol. Chem. 2000
6146630 An enzyme that removes clathrin coats: purification of an uncoating ATPase

Braell, WA, Schlossman, DM, Rothman, JE, Schmid, SL

J. Cell Biol. 1984
26042225 The role of molecular chaperones in clathrin mediated vesicular trafficking

Lafer, EM, Sousa, R

Front Mol Biosci 2015
8524399 Role of auxilin in uncoating clathrin-coated vesicles

Holstein, SE, Eisenberg, E, Greene, LE, Martin, B, Lindner, R, Ungewickell, H, Ungewickell, E, Barouch, W, Prasad, K

Nature 1995
17978091 A motif in the clathrin heavy chain required for the Hsc70/auxilin uncoating reaction

Yu, A, Böcking, T, Kirchhausen, T, Boll, W, Rapoport, I

Mol. Biol. Cell 2008
20826345 Structure of the PTEN-like region of auxilin, a detector of clathrin-coated vesicle budding

Kirchhausen, T, Han, D, Guan, R, Harrison, SC, Dai, H

Structure 2010
15502813 Structure of an auxilin-bound clathrin coat and its implications for the mechanism of uncoating

Kirchhausen, T, Cheng, Y, Harrison, SC, Walz, T, Fotin, A, Grigorieff, N

Nature 2004
21445324 A high precision survey of the molecular dynamics of mammalian clathrin-mediated endocytosis

Merrifield, CJ, Taylor, MJ, Perrais, D

PLoS Biol. 2011
20033059 Structure of clathrin coat with bound Hsc70 and auxilin: mechanism of Hsc70-facilitated disassembly

Böcking, T, Xing, Y, Kirchhausen, T, Harrison, SC, Wolf, M, Grigorieff, N

EMBO J. 2010
15502812 Molecular model for a complete clathrin lattice from electron cryomicroscopy

Kirchhausen, T, Cheng, Y, Walz, T, Harrison, SC, Sliz, P, Fotin, A, Grigorieff, N

Nature 2004
16798879 A burst of auxilin recruitment determines the onset of clathrin-coated vesicle uncoating

Kirchhausen, T, Massol, RH, Boll, W, Griffin, AM

Proc. Natl. Acad. Sci. U.S.A. 2006
11470803 Multiple interactions of auxilin 1 with clathrin and the AP-2 adaptor complex

Scheele, U, Ungewickell, E, Kalthoff, C

J. Biol. Chem. 2001
Orthologous Events
Cite Us!