HSPA8-mediated ATP hydrolysis promotes vesicle uncoating

Stable Identifier
Reaction [transition]
Homo sapiens
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HSPA8 hydrolyzes ATP to promote dissociation of the clathrin coat from the vesicle (reviewed in Sousa and Lafer, 2015). Interaction of HSPA8 with the C-terminal tail of clathrin may sterically block re-stabilization of the clathrin coat, which is thought to undergo transient cycles of 'breathing', or loosening of the interactions between the triskelions (Barouch et al, 1997; Rapoport et al, 2008; Xing et al, 2010). Alternately, HSPA8 may destabilize the clathrin coat through intermolecular collisions with the coat (reveiwed in Sousa and Lafer, 2015). The HSPA8-clathrin interaction persists once clathrin has been removed from the vesicle. This is thought to preclude aberrant repolymerization of clathrin by sequestering free clathrin (Schlossman et al, 1984; reviewed in Sousa and Lafer, 2015).

Literature References
PubMed ID Title Journal Year
9100026 Auxilin-induced interaction of the molecular chaperone Hsc70 with clathrin baskets

Barouch, W, Prasad, K, Greene, L, Eisenberg, E

Biochemistry 1997
17978091 A motif in the clathrin heavy chain required for the Hsc70/auxilin uncoating reaction

Rapoport, I, Boll, W, Yu, A, Böcking, T, Kirchhausen, T

Mol. Biol. Cell 2008
6146630 An enzyme that removes clathrin coats: purification of an uncoating ATPase

Schlossman, DM, Schmid, SL, Braell, WA, Rothman, JE

J. Cell Biol. 1984
26042225 The role of molecular chaperones in clathrin mediated vesicular trafficking

Sousa, R, Lafer, EM

Front Mol Biosci 2015
20033059 Structure of clathrin coat with bound Hsc70 and auxilin: mechanism of Hsc70-facilitated disassembly

Xing, Y, Böcking, T, Wolf, M, Grigorieff, N, Kirchhausen, T, Harrison, SC

EMBO J. 2010
Catalyst Activity

ATPase activity of PI(4)P:p-T156 AP-2:clathrin:ITSNs:EPS15:REPS1:SGIP1:NECAPs:AAK1:CLASP proteins:cargo:F-BAR proteins:BAR domain proteins:ARP2/3 complex:WASL:f-actin:HIP dimers:DNM:GDP:SYNJs:auxilin:HSPA8:ATP [clathrin-coated endocytic vesicle membrane]

Orthologous Events
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