RNF5 and RNF185 ubiquitinate CFTR F508del as part of the retrotranslocon that targets the receptor for degradation through the ERAD pathway. Both depletion of the E3 ligases by siRNA and expression of a catalytically inactive form of the enzyme strongly inhibits CFTR degradation (El Khouri et al, 2013; Younger et al, 2006).
El Khouri, E,
Le Pavec, G,
ubiquitin protein ligase activity of CFTR F508del:ERAD complex [endoplasmic reticulum membrane]
Gain of function of CFTR F508del:ERAD complex [endoplasmic reticulum membrane]