Reactome | UBA6 conjugates ubiquitin to UBA6 in the cytosol

UBA6 conjugates ubiquitin to UBA6 in the cytosol

Stable Identifier

R-HSA-8865098

Type

Reaction [transition]

Species

Homo sapiens

Compartment

cytosol

ReviewStatus

5/5

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UBA6 conjugates ubiquitin to UBA6 in the cytosol

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As inferred from the homologous UBA1, activation of ubiquitin by UBA6 (UBE1L1) proceeds through 3 steps: adenylation of ubiquitin, conjugation of ubiquitin from adenyl-ubiquitin to an internal cysteine residue of UBA6, and adenylation of a second molecule of ubiquitin. In the second step, the thiol group of an internal cysteine residue of UBA6 attacks the acyl-adenyl bond of the C-terminal glycine of adenylated ubiquitin, resulting in a thioester bond between the cysteine residue and the C-terminal glycine of ubiquitin (Jin et al. 2007, Pelzer et al. 2007, reviewed in Groettrup et al. 2008).

Literature References

PubMed ID	Title	Journal	Year
17597759	Dual E1 activation systems for ubiquitin differentially regulate E2 enzyme charging Jin, J, Li, X, Gygi, SP, Harper, JW	Nature	2007
18353650	Activating the ubiquitin family: UBA6 challenges the field Groettrup, M, Pelzer, C, Schmidtke, G, Hofmann, K	Trends Biochem. Sci.	2008
17580310	UBE1L2, a novel E1 enzyme specific for ubiquitin Pelzer, C, Kassner, I, Matentzoglu, K, Singh, RK, Wollscheid, HP, Scheffner, M, Schmidtke, G, Groettrup, M	J. Biol. Chem.	2007