Reactome | CL-LK binds carbohydrates on target cell surface

CL-LK binds carbohydrates on target cell surface

Stable Identifier

R-HSA-8852509

Type

Reaction [binding]

Species

Homo sapiens

Compartment

extracellular region, plasma membrane

ReviewStatus

5/5

Locations in the PathwayBrowser

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Immune System (Homo sapiens)

- Innate Immune System (Homo sapiens)
  - Complement cascade (Homo sapiens)
    - Initial triggering of complement (Homo sapiens)
      - Creation of C4 and C2 activators (Homo sapiens)
        
        Lectin pathway of complement activation (Homo sapiens)
        
        CL-LK binds carbohydrates on target cell surface (Homo sapiens)

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CL-LK binds carbohydrates on target cell surface

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Collectin kidney 1 (CL-K1, CL-11, COLEC11) (Keshi et al. 2006) forms disulfide-bridged stable heteromers with collectin liver 1 (CL-L1, COLEC10) (Otahani et al. 1999), with a ratio of one COLEC10 to two COLEC11 polypeptide chains. The majority of plasma COLEC11 was found in complex with COLEC10 (Henriksen et al. 2013). The resulting COLEC10:2xCOLEC11 heterocomplex, termred CL-LK, contains multiple Ca2+ -dependent carbohydrate-recognition domains (CRDs) and collagen-like regions, which allow high-avidity binding (KD ~10-9 M) to target cell surface carbohydrate patterns (Bajic et al. 2015); COLEC11 recognizes L-fucose and D-mannose and the disaccharide D-mannose(alpha1-2)-D-mannose (Keshi et al. 2006, Hansen et al. 2010, Selman & Hansen 2012, ). CL-LK can bind mannose-rich patterns on M. tuberculosis (Troegeler et al. 2015). The CL-LK complex was able to bind mannan-binding lectin-associated serine proteases (MASPs) in vitro with affinities in the nM range, and was associated with MASP1/3 and MASP2 in plasma. Upon binding to mannan or DNA in the presence of MASP2, the COLEC10:COLEC11 complex mediated deposition of C4b (Henriksen et al. 2013). Polymorphisms in the COLEC11 gene cause 3MC syndrome (Rooryck et al. 2011).

Literature References

PubMed ID	Title	Journal	Year
26173080	Collectin CL-LK Is a Novel Soluble Pattern Recognition Receptor for Mycobacterium tuberculosis Rasolofo, V, Nigou, J, Hansen, S, Mercier, I, Duval, C, Troegeler, A, Mori, K, Lugo-Villarino, G, Hudrisier, D, Ohtani, K, Bénard, A, Neyrolles, O, Wakamiya, N, Henriksen, ML	PLoS ONE	2015