UBA1 conjugates ubiquitin to UBA1 in the nucleus

Stable Identifier
R-HSA-8852136
Type
Reaction [dissociation]
Species
Homo sapiens
Compartment
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UBA1 is located in both the nucleus and cytoplasm (Grenfell 1994). Activation of ubiquitin by UBA1 proceeds through 3 steps: adenylation of ubiquitin, conjugation of ubiquitin from adenyl-ubiquitin to an internal cysteine residue of UBA1, and adenylation of a second molecule of ubiquitin. In the second step, the thiol group of an internal cysteine residue of UBA1 attacks the acyl-adenyl bond of the C-terminal glycine of adenylated ubiquitin, resulting in a thioester bond between the cysteine residue and the C-terminal glycine of ubiquitin (Jin et al. 2007, inferred from the rabbit homologue in Haas et al. 1982, Hershko et al. 1983, reviewed in Groettrup et al. 2008).

Literature References
PubMed ID Title Journal Year
18353650 Activating the ubiquitin family: UBA6 challenges the field

Groettrup, M, Pelzer, C, Schmidtke, G, Hofmann, K

Trends Biochem. Sci. 2008
17597759 Dual E1 activation systems for ubiquitin differentially regulate E2 enzyme charging

Jin, J, Li, X, Gygi, SP, Harper, JW

Nature 2007
6277905 Ubiquitin-activating enzyme. Mechanism and role in protein-ubiquitin conjugation

Haas, AL, Warms, JV, Hershko, A, Rose, IA

J. Biol. Chem. 1982
6305978 Components of ubiquitin-protein ligase system. Resolution, affinity purification, and role in protein breakdown

Hershko, A, Heller, H, Elias, S, Ciechanover, A

J. Biol. Chem. 1983
8010951 Nuclear localization of the ubiquitin-activating enzyme, E1, is cell-cycle-dependent

Grenfell, SJ, Trausch-Azar, JS, Handley-Gearhart, PM, Ciechanover, A, Schwartz, AL

Biochem. J. 1994
Participants
Participant Of
Orthologous Events
Authored
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Created