Reactome | UBA1 conjugates ubiquitin to nuclear E2 enzymes

UBA1 conjugates ubiquitin to nuclear E2 enzymes

Stable Identifier

R-HSA-8852130

Type

Reaction [transition]

Species

Homo sapiens

Compartment

nucleoplasm

ReviewStatus

5/5

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UBA1 conjugates ubiquitin to nuclear E2 enzymes

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In the nucleus (Grenfell et al. 1994), the UBA1-ubiquitin thiol ester conjugate transfers ubiquitin from UBA1 to an internal cysteine residue of the E2 enzyme, forming a thiol ester conjugate between ubiquitin and the cysteine residue of E2 (Jin et al. 2007, inferred from the rabbit homologue in Hershko et al. 1983, reviewed in Groettrup et al. 2008). The E2 then disengages from UBA1 (Eletr et al. 2005).

Literature References

PubMed ID	Title	Journal	Year
17597759	Dual E1 activation systems for ubiquitin differentially regulate E2 enzyme charging Jin, J, Li, X, Gygi, SP, Harper, JW	Nature	2007
18353650	Activating the ubiquitin family: UBA6 challenges the field Groettrup, M, Pelzer, C, Schmidtke, G, Hofmann, K	Trends Biochem. Sci.	2008
6305978	Components of ubiquitin-protein ligase system. Resolution, affinity purification, and role in protein breakdown Hershko, A, Heller, H, Elias, S, Ciechanover, A	J. Biol. Chem.	1983
8010951	Nuclear localization of the ubiquitin-activating enzyme, E1, is cell-cycle-dependent Grenfell, SJ, Trausch-Azar, JS, Handley-Gearhart, PM, Ciechanover, A, Schwartz, AL	Biochem. J.	1994
16142244	E2 conjugating enzymes must disengage from their E1 enzymes before E3-dependent ubiquitin and ubiquitin-like transfer Eletr, ZM, Huang, DT, Duda, DM, Schulman, BA, Kuhlman, B	Nat. Struct. Mol. Biol.	2005