Reactome | UBA1 conjugates ubiquitin to cytosolic E2 enzymes

UBA1 conjugates ubiquitin to cytosolic E2 enzymes

Stable Identifier

R-HSA-8852129

Type

Reaction [transition]

Species

Homo sapiens

Compartment

cytosol

ReviewStatus

5/5

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UBA1 conjugates ubiquitin to cytosolic E2 enzymes

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In the cytosol, the UBA1-ubiquitin thiol ester conjugate transfers ubiquitin from UBA1 to an internal cysteine residue of the E2 enzyme, forming a thiol ester conjugate between ubiquitin and the cysteine residue of E2 (Jin et al. 2007, inferred from the rabbit homologue in Hershko et al. 1983). The E2 then disengages from UBA1 (Eletr et al. 2005).

Literature References

PubMed ID	Title	Journal	Year
17597759	Dual E1 activation systems for ubiquitin differentially regulate E2 enzyme charging Li, X, Gygi, SP, Jin, J, Harper, JW	Nature	2007
6305978	Components of ubiquitin-protein ligase system. Resolution, affinity purification, and role in protein breakdown Ciechanover, A, Elias, S, Heller, H, Hershko, A	J. Biol. Chem.	1983
16142244	E2 conjugating enzymes must disengage from their E1 enzymes before E3-dependent ubiquitin and ubiquitin-like transfer Huang, DT, Kuhlman, B, Duda, DM, Schulman, BA, Eletr, ZM	Nat. Struct. Mol. Biol.	2005