Reactome | UBA1 adenylates ubiquitin in the nucleoplasm

UBA1 adenylates ubiquitin in the nucleoplasm

Stable Identifier

R-HSA-8852128

Type

Reaction [binding]

Species

Homo sapiens

Compartment

nucleoplasm

ReviewStatus

5/5

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UBA1 adenylates ubiquitin in the nucleoplasm

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UBA1 is present in both the cytosol and nucleoplasm (Grenfell et al. 1994). Activation of ubiquitin by UBA1 proceeds through 3 steps: adenylation of ubiquitin, conjugation of ubiquitin from adenyl-ubiquitin to an internal cysteine residue of UBA1, and adenylation of a second molecule of ubiquitin. In the first step, the adenylation module of UBA1 catalyzes the acyl-adenylation of the C-terminal glycine of ubiquitin (Jin et al. 2007, inferred from the rabbit homologue in Haas et al. 1982, Hershko et al. 1983, reviewed in Groettrup et al. 2008).

Literature References

PubMed ID	Title	Journal	Year
17597759	Dual E1 activation systems for ubiquitin differentially regulate E2 enzyme charging Li, X, Gygi, SP, Jin, J, Harper, JW	Nature	2007
18353650	Activating the ubiquitin family: UBA6 challenges the field Hofmann, K, Pelzer, C, Groettrup, M, Schmidtke, G	Trends Biochem. Sci.	2008
6277905	Ubiquitin-activating enzyme. Mechanism and role in protein-ubiquitin conjugation Rose, IA, Hershko, A, Haas, AL, Warms, JV	J. Biol. Chem.	1982
6305978	Components of ubiquitin-protein ligase system. Resolution, affinity purification, and role in protein breakdown Ciechanover, A, Elias, S, Heller, H, Hershko, A	J. Biol. Chem.	1983
8010951	Nuclear localization of the ubiquitin-activating enzyme, E1, is cell-cycle-dependent Schwartz, AL, Ciechanover, A, Grenfell, SJ, Handley-Gearhart, PM, Trausch-Azar, JS	Biochem. J.	1994