ARHGAP35 (p190 Rho GTPase activating protein) binds GTP-bound RHOA (Li et al. 1997). Phosphorylation of ARHGAP35 by SRC family kinases (Holinstat et al. 2006), including PTK6 (Shen et al. 2008), is necessary for ARHGAP35-mediated activation of Rho GTPase activity. It is uncertain whether phosphorylation of ARHGAP35 by SRC family kinases is necessary for binding to RHOA.