Addition of nucleotides 10 and 11 on the growing transcript: Third Transition

Stable Identifier
Reaction [transition]
Homo sapiens
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Formation of phosphodiester bonds nine and ten creates RNA products, which do not dissociate from the RNA pol II initiation complex. The transcription complex has enter the productive elongation phase. TFIIH and ATP-hydrolysis are required for efficient promoter escape. The open region (“transcription bubble”) expands concomitant with the site of RNA-extension. The region upstream from the transcription start site (-9 to -3) collapses to the double-stranded state. TFIIH remains associated to the RNA pol II initiation complex.

Literature References
PubMed ID Title Journal Year
7601352 Recycling of the general transcription factors during RNA polymerase II transcription.

Zawel, L, Kumar, KP, Reinberg, D

Genes Dev 1995
9405375 Three transitions in the RNA polymerase II transcription complex during initiation.

Fiedler, U, Timmers, HT, Holstege, FC

EMBO J 1998
Catalyst Activity

DNA-directed 5'-3' RNA polymerase activity of RNA Polymerase II (unphosphorylated):TFIIF complex [nucleoplasm]

Orthologous Events
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