Reactome | Phosphorylation of Cdc25C at Ser 216 by Chk1

Phosphorylation of Cdc25C at Ser 216 by Chk1

Stable Identifier

R-HSA-75010

Type

Reaction [transition]

Species

Homo sapiens

Compartment

nucleoplasm

ReviewStatus

5/5

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Phosphorylation of Cdc25C at Ser 216 by Chk1

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A recombinant human CHEK1 forms a complex with and phosphorylates a recmbinant human CDC25C on serine residue S216 (Sanchez et al. 1997). Phosphorylation of CDC25C at S216 results in both the inhibition of CDC25C phosphatase activity and the creation of a 14-3-3 docking site (Peng et al. 1997). Ectopic overexpression of the human CDC25C S216A mutant reduces the ability of cells to arrest in G2 in response to DNA damage (Sanchez et al. 1997).

Literature References

PubMed ID	Title	Journal	Year
9278511	Conservation of the Chk1 checkpoint pathway in mammals: linkage of DNA damage to Cdk regulation through Cdc25 Sanchez, Y, Piwnica-Worms, H, Richman, R, Thoma, RS, Elledge, SJ, Wu, Z, Wong, C	Science	1997
9278512	Mitotic and G2 checkpoint control: regulation of 14-3-3 protein binding by phosphorylation of Cdc25C on serine-216. Piwnica-Worms, H, Shaw, AS, Thoma, RS, Peng, CY, Wu, Z, Graves, PR	Science	1997