Phosphorylation of Cdc25C at Ser 216 by Chk1

Stable Identifier
R-HSA-75010
Type
Reaction [transition]
Species
Homo sapiens
Compartment
ReviewStatus
5/5
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A recombinant human CHEK1 forms a complex with and phosphorylates a recmbinant human CDC25C on serine residue S216 (Sanchez et al. 1997). Phosphorylation of CDC25C at S216 results in both the inhibition of CDC25C phosphatase activity and the creation of a 14-3-3 docking site (Peng et al. 1997). Ectopic overexpression of the human CDC25C S216A mutant reduces the ability of cells to arrest in G2 in response to DNA damage (Sanchez et al. 1997).
Literature References
PubMed ID Title Journal Year
9278511 Conservation of the Chk1 checkpoint pathway in mammals: linkage of DNA damage to Cdk regulation through Cdc25

Sanchez, Y, Piwnica-Worms, H, Richman, R, Thoma, RS, Elledge, SJ, Wu, Z, Wong, C

Science 1997
9278512 Mitotic and G2 checkpoint control: regulation of 14-3-3 protein binding by phosphorylation of Cdc25C on serine-216.

Piwnica-Worms, H, Shaw, AS, Thoma, RS, Peng, CY, Wu, Z, Graves, PR

Science 1997
Participants
Participates
Catalyst Activity

protein serine/threonine kinase activity of p-S317,S345-CHEK1 [nucleoplasm]

Orthologous Events
Authored
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