GAR + 10-Formyl-THF => FGAR + THF

Stable Identifier
R-HSA-73813
Type
Reaction [transition]
Species
Homo sapiens
Compartment
Synonyms
5-phosphoribosylglycinamide (GAR) + 10-formyl-tetrahydrofolate => 5'-phosphoribosylformylglycinamide (FGAR) + tetrahydrofolate
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The irreversible transfer of a formyl group to cytosolic 5-phosphoribosylglycinamide (GAR) to form 5'-phosphoribosylformylglycinamide (FGAR) is catalyzed by the phosphoribosylglycinamide formyltransferase domain of the trifunctional protein, "phosphoribosylglycinamide formyltransferase, phosphoribosylglycinamide synthetase, phosphoribosylaminoimidazole synthetase" (GART) (Aimi et al. 1990; Zhang et al. 2002). Fluoresence microscopy studies of cultured human cells have shown that GART is cytosolic and suggest that it may co-localize with other enzymes of de novo IMP biosynthesis under some metabolic conditions (Gooljarsingh et al. 2001; An et al. 2008).

Literature References
PubMed ID Title Journal Year
12450384 Crystal structures of human GAR Tfase at low and high pH and with substrate beta-GAR

Zhang, Y, Desharnais, J, Greasley, SE, Beardsley, GP, Boger, DL, Wilson, IA

Biochemistry 2002
2147474 De novo purine nucleotide biosynthesis: cloning of human and avian cDNAs encoding the trifunctional glycinamide ribonucleotide synthetase-aminoimidazole ribonucleotide synthetase-glycinamide ribonucleotide transformylase by functional complementation in E. coli

Aimi, J, Qiu, H, Williams, J, Zalkin, H, Dixon, JE

Nucleic Acids Res 1990
18388293 Reversible compartmentalization of de novo purine biosynthetic complexes in living cells

An, S, Kumar, R, Sheets, ED, Benkovic, SJ

Science 2008
11381136 Localization of GAR transformylase in Escherichia coli and mammalian cells

Gooljarsingh, LT, Ramcharan, J, Gilroy, S, Benkovic, SJ

Proc Natl Acad Sci USA 2001
Participants
Participant Of
Catalyst Activity
Catalyst Activity
Title
phosphoribosylglycinamide formyltransferase activity of GART [cytosol]
Physical Entity
Activity
Orthologous Events
Cross References
Rhea
Authored
Created