GAR + 10-Formyl-THF => FGAR + THF

Stable Identifier
Reaction [transition]
Homo sapiens
5-phosphoribosylglycinamide (GAR) + 10-formyl-tetrahydrofolate => 5'-phosphoribosylformylglycinamide (FGAR) + tetrahydrofolate
Locations in the PathwayBrowser
SVG |   | PPTX  | SBGN
Click the image above or here to open this reaction in the Pathway Browser
The layout of this reaction may differ from that in the pathway view due to the constraints in pathway layout

The irreversible transfer of a formyl group to cytosolic 5-phosphoribosylglycinamide (GAR) to form 5'-phosphoribosylformylglycinamide (FGAR) is catalyzed by the phosphoribosylglycinamide formyltransferase domain of the trifunctional protein, "phosphoribosylglycinamide formyltransferase, phosphoribosylglycinamide synthetase, phosphoribosylaminoimidazole synthetase" (GART) (Aimi et al. 1990; Zhang et al. 2002). Fluoresence microscopy studies of cultured human cells have shown that GART is cytosolic and suggest that it may co-localize with other enzymes of de novo IMP biosynthesis under some metabolic conditions (Gooljarsingh et al. 2001; An et al. 2008).

Literature References
PubMed ID Title Journal Year
2147474 De novo purine nucleotide biosynthesis: cloning of human and avian cDNAs encoding the trifunctional glycinamide ribonucleotide synthetase-aminoimidazole ribonucleotide synthetase-glycinamide ribonucleotide transformylase by functional complementation in E. coli

Qiu, H, Zalkin, H, Aimi, J, Williams, J, Dixon, JE

Nucleic Acids Res 1990
12450384 Crystal structures of human GAR Tfase at low and high pH and with substrate beta-GAR

Beardsley, GP, Boger, DL, Desharnais, J, Greasley, SE, Zhang, Y, Wilson, IA

Biochemistry 2002
18388293 Reversible compartmentalization of de novo purine biosynthetic complexes in living cells

Benkovic, SJ, Sheets, ED, Kumar, R, An, S

Science 2008
11381136 Localization of GAR transformylase in Escherichia coli and mammalian cells

Benkovic, SJ, Gilroy, S, Gooljarsingh, LT, Ramcharan, J

Proc Natl Acad Sci USA 2001
Catalyst Activity

phosphoribosylglycinamide formyltransferase activity of GART [cytosol]

Orthologous Events
Cross References
Cite Us!