Reactome | GAR + 10-Formyl-THF => FGAR + THF

GAR + 10-Formyl-THF => FGAR + THF

Stable Identifier

R-HSA-73813

Type

Reaction [transition]

Species

Homo sapiens

Compartment

cytosol

Synonyms

5-phosphoribosylglycinamide (GAR) + 10-formyl-tetrahydrofolate => 5'-phosphoribosylformylglycinamide (FGAR) + tetrahydrofolate

ReviewStatus

5/5

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The irreversible transfer of a formyl group to cytosolic 5-phosphoribosylglycinamide (GAR) to form 5'-phosphoribosylformylglycinamide (FGAR) is catalyzed by the phosphoribosylglycinamide formyltransferase domain of the trifunctional protein, "phosphoribosylglycinamide formyltransferase, phosphoribosylglycinamide synthetase, phosphoribosylaminoimidazole synthetase" (GART) (Aimi et al. 1990; Zhang et al. 2002). Fluoresence microscopy studies of cultured human cells have shown that GART is cytosolic and suggest that it may co-localize with other enzymes of de novo IMP biosynthesis under some metabolic conditions (Gooljarsingh et al. 2001; An et al. 2008).

Literature References

PubMed ID	Title	Journal	Year
2147474	De novo purine nucleotide biosynthesis: cloning of human and avian cDNAs encoding the trifunctional glycinamide ribonucleotide synthetase-aminoimidazole ribonucleotide synthetase-glycinamide ribonucleotide transformylase by functional complementation in E. coli Qiu, H, Zalkin, H, Aimi, J, Williams, J, Dixon, JE	Nucleic Acids Res	1990
12450384	Crystal structures of human GAR Tfase at low and high pH and with substrate beta-GAR Beardsley, GP, Boger, DL, Desharnais, J, Greasley, SE, Zhang, Y, Wilson, IA	Biochemistry	2002
18388293	Reversible compartmentalization of de novo purine biosynthetic complexes in living cells Benkovic, SJ, Sheets, ED, Kumar, R, An, S	Science	2008
11381136	Localization of GAR transformylase in Escherichia coli and mammalian cells Benkovic, SJ, Gilroy, S, Gooljarsingh, LT, Ramcharan, J	Proc Natl Acad Sci USA	2001