FGAR + L-Glutamine + ATP + H2O => FGAM + L-Glutamate + ADP + Pi

Stable Identifier
Reaction [transition]
Homo sapiens
5'-phosphoribosylformylglycinamide (FGAR) + L-glutamine + ATP + H2O => 5'-phosphoribosylformylglycinamidine (FGAM) + L-glutamate + adenosine 5'-diphosphate + orthophosphate
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The irreversible transfer of an amino group from L-glutamine to 5'-phosphoribosylformylglycinamide (FGAR), forming 5'-phosphoribosylformylglycinamidine (FGAM) and glutamate, accompanied by the conversion of ATP to ADP and orthophosphate, is catalyzed by phosphoribosylformylglycinamidine synthetase. The human enzyme has been purified and characterized biochemically (Barnes et al. 1994). Fluoresence microscopy studies of cultured human cells have shown that the enzyme is cytosolic and suggest that it may co-localize with other enzymes of de novo IMP biosynthesis under some metabolic conditions (An et al. 2008).

Literature References
PubMed ID Title Journal Year
18388293 Reversible compartmentalization of de novo purine biosynthetic complexes in living cells

Benkovic, SJ, Sheets, ED, Kumar, R, An, S

Science 2008
8110788 Purification of, generation of monoclonal antibodies to, and mapping of phosphoribosyl N-formylglycinamide amidotransferase

Patterson, D, Walton, KA, Barton, JW, Hart, IM, Bleskan, JH, Barnes, TS

Biochemistry 1994
Catalyst Activity

phosphoribosylformylglycinamidine synthase activity of PFAS [cytosol]

Orthologous Events
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