Reactome | hydrolysis of 2'-deoxyuridine 5'-triphosphate to form 2'-deoxyuridine 5'-phosphate

hydrolysis of 2'-deoxyuridine 5'-triphosphate to form 2'-deoxyuridine 5'-phosphate

Stable Identifier

R-HSA-73666

Type

Reaction [transition]

Species

Homo sapiens

Compartment

nucleoplasm

Synonyms

dUTP + H2O => dUMP + diphosphate + H(+), dUTP + H2O => dUMP + pyrophosphate

ReviewStatus

5/5

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hydrolysis of 2'-deoxyuridine 5'-triphosphate to form 2'-deoxyuridine 5'-phosphate

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Deoxyuridine triphosphatase (DUT) catalyzes the hydrolysis of dUTP to form dUMP and pyrophosphate. Two isoforms of DUT are expressed, generated by alternative splicing. The major one, annotated here, is localized to the nucleoplasm (Ladner et al. 1996). The enzyme is a homotrimer (Mol et al. 1996). In the cell, this reaction depletes the supply of dUTP, preventing its incorporation into DNA, while generating dUMP, the immediate precursor of thymidine nucleotides.

Literature References

PubMed ID	Title	Journal	Year
8631816	Characterization of distinct nuclear and mitochondrial forms of human deoxyuridine triphosphate nucleotidohydrolase Ladner, RD, McNulty, DE, Carr, SA, Roberts, GD, Caradonna, SJ	J Biol Chem	1996
8805593	Human dUTP pyrophosphatase: uracil recognition by a beta hairpin and active sites formed by three separate subunits Mol, CD, Harris, JM, McIntosh, EM, Tainer, JA	Structure	1996