dUTP + H2O => dUMP + pyrophosphate

Stable Identifier
Reaction [transition]
Homo sapiens
hydrolysis of 2'-deoxyuridine 5'-triphosphate to form 2'-deoxyuridine 5'-phosphate
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Deoxyuridine triphosphatase (DUT) catalyzes the hydrolysis of dUTP to form dUMP and pyrophosphate. Two isoforms of DUT are expressed, generated by alternative splicing. The major one, annotated here, is localized to the nucleoplasm (Ladner et al. 1996). The enzyme is a homotrimer (Mol et al. 1996). In the cell, this reaction depletes the supply of dUTP, preventing its incorporation into DNA, while generating dUMP, the immediate precursor of thymidine nucleotides.

Literature References
PubMed ID Title Journal Year
8631816 Characterization of distinct nuclear and mitochondrial forms of human deoxyuridine triphosphate nucleotidohydrolase

Roberts, GD, Ladner, RD, Caradonna, SJ, McNulty, DE, Carr, SA

J Biol Chem 1996
8805593 Human dUTP pyrophosphatase: uracil recognition by a beta hairpin and active sites formed by three separate subunits

Tainer, JA, Mol, CD, McIntosh, EM, Harris, JM

Structure 1996
Catalyst Activity

dUTP diphosphatase activity of DUT trimer [nucleoplasm]

Orthologous Events
Cross References
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