N-carbamoyl L-aspartate + H+ <=> (S)-dihydroorotate + H2O

Stable Identifier
R-HSA-73571
Type
Reaction [transition]
Species
Homo sapiens
Compartment
Synonyms
condensation of N-Carbamoyl-L-aspartate to form (S)-Dihydroorotate (ring closure)
Locations in the PathwayBrowser
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The synthesis of dihydroorotate from N-carbamoyl L-aspartate is catalyzed by the dihydroorotase activity of cytosolic trifunctional CAD (carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase) protein. This activity has not been directly demonstrated in experimental studies of the purified human protein, but has been inferred from the behavior of the purified hamster protein and the high degree of sequence similarity between the cloned hamster and human genes (Iwahana et al. 1996). Also on the basis of this similarity, the active human protein is annotated as a hexamer (Lee et al. 1985).

Literature References
PubMed ID Title Journal Year
8619816 Molecular cloning of a human cDNA encoding a trifunctional enzyme of carbamoyl-phosphate synthetase-aspartate transcarbamoylase-dihydroorotase in de Novo pyrimidine synthesis

Iwahana, H, Fujimura, H, Ii, S, Kondo, M, Moritani, M, Takahashi, Y, Yamaoka, T, Yoshimoto, K, Itakura, M

Biochem Biophys Res Commun 1996
Participants
Participant Of
Catalyst Activity
Catalyst Activity
Title
dihydroorotase activity of CAD hexamer [cytosol]
Physical Entity
Activity
Orthologous Events
Cross References
Rhea
Authored
Created