Internal Methylation of mRNA

Stable Identifier
Reaction [transition]
Homo sapiens
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In addition to the methylation of the 5'-cap, there is methylation of internal nucleotides in the mRNA. This methylation can occur in translated and untranslated regions. One to three methyl groups have been seen per mRNA molecule, but methylation is non-stoichiometric. The most frequent methylation observed is at the N6 position of adenosine. The function of mRNA internal methylation, if any, is unknown.
Literature References
PubMed ID Title Journal Year
24407421 Mammalian WTAP is a regulatory subunit of the RNA N6-methyladenosine methyltransferase

Sun, BF, Adhikari, S, Yuan, WP, Wang, WJ, Liu, X, Dahal, U, Yang, X, Yang, YG, Chen, YS, Lv, Y, Zhao, X, Wang, X, Lou, XM, Huang, J, Zhao, YL, Ping, XL, Liu, F, Xiao, W, Rendtlew Danielsen, JM, Zhu, XF, Li, A, Yang, Y, Wang, L, Cheng, T, Shi, Y

Cell Res. 2014
24981863 Perturbation of m6A writers reveals two distinct classes of mRNA methylation at internal and 5' sites

Bushkin, GG, Mumbach, MR, Satija, R, Wang, T, Pacold, ME, Sanjana, NE, Hacohen, N, Carr, SA, Freinkman, E, Regev, A, Cacchiarelli, D, Jovanovic, M, Maciag, K, Habib, N, Ter-Ovanesyan, D, Mertins, P, Lander, ES, Schwartz, S, Zhang, F, Mikkelsen, TS

Cell Rep 2014
24316715 A METTL3-METTL14 complex mediates mammalian nuclear RNA N6-adenosine methylation

Lu, Z, Wang, X, Zhang, L, Yue, Y, Chen, W, Fu, Y, Jia, G, Yu, M, Dai, Q, He, C, Han, D, Deng, X, Liu, J

Nat. Chem. Biol. 2014
9409616 Purification and cDNA cloning of the AdoMet-binding subunit of the human mRNA (N6-adenosine)-methyltransferase.

Matera, AG, Rottman, FM, Shambaugh, ME, Polayes, D, Bokar, JA

RNA 1998
Catalyst Activity

mRNA (N6-adenosine)-methyltransferase activity of mRNA (N6-adenosine)-methyltransferase [nucleoplasm]

Orthologous Events
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