Oxaloacetate + NADH + H+ <=> (S)-Malate + NAD+

Stable Identifier
R-HSA-71783
Type
Reaction [transition]
Species
Homo sapiens
Compartment
mitochondrial matrix
Locations in the PathwayBrowser
Expand all
General
SVG | 
PNG
Low
Medium
High
 | PPTX  | SBGN
Oxaloacetate + NADH + H+ <=> (S)-Malate + NAD+
Click the image above or here to open this reaction in the Pathway Browser
The layout of this reaction may differ from that in the pathway view due to the constraints in pathway layout

Mitochondrial malate dehydrogenase catalyzes the reversible reaction of oxaloacetate and NADH + H+ to form malate and NAD+ (Luo et al. 2006). Unpublished crystallographic data indicate that the protein is a dimer (PDB 3E04).

Literature References
PubMed ID Title Journal Year
16740313 An NADH-tetrazolium-coupled sensitive assay for malate dehydrogenase in mitochondria and crude tissue homogenates

Luo, C, Wang, X, Long, J, Liu, J

J Biochem Biophys Methods 2006
Participants
Input
Output
Participant Of
hasEvent
Event Information
Reverse Reaction
(S)-Malate + NAD+ <=> Oxaloacetate + NADH + H+ (Homo sapiens)
Catalyst Activity
Catalyst Activity
Title
L-malate dehydrogenase activity of MDH2 dimer [mitochondrial matrix]
Physical Entity
MDH2 dimer [mitochondrial matrix]
Activity
L-malate dehydrogenase activity (0030060)
Orthologous Events
Oxaloacetate + NADH + H+ <=> (S)-Malate + NAD+ (Bos taurus)
Oxaloacetate + NADH + H+ <=> (S)-Malate + NAD+ (Caenorhabditis elegans)
Oxaloacetate + NADH + H+ <=> (S)-Malate + NAD+ (Canis familiaris)
Oxaloacetate + NADH + H+ <=> (S)-Malate + NAD+ (Danio rerio)
Oxaloacetate + NADH + H+ <=> (S)-Malate + NAD+ (Drosophila melanogaster)
Oxaloacetate + NADH + H+ <=> (S)-Malate + NAD+ (Gallus gallus)
Oxaloacetate + NADH + H+ <=> (S)-Malate + NAD+ (Mus musculus)
Oxaloacetate + NADH + H+ <=> (S)-Malate + NAD+ (Plasmodium falciparum)
Oxaloacetate + NADH + H+ <=> (S)-Malate + NAD+ (Rattus norvegicus)
Oxaloacetate + NADH + H+ <=> (S)-Malate + NAD+ (Saccharomyces cerevisiae)
Oxaloacetate + NADH + H+ <=> (S)-Malate + NAD+ (Schizosaccharomyces pombe)
Oxaloacetate + NADH + H+ <=> (S)-Malate + NAD+ (Xenopus tropicalis)
oxaloacetate + NADH + H+ <=> malate + NAD+ (Gallus gallus)
Cross References
Rhea
Reviewed
Harris, RA  (2008-09-10)