(S)-Malate + NAD+ <=> Oxaloacetate + NADH + H+

Stable Identifier
R-HSA-70979
Type
Reaction [transition]
Species
Homo sapiens
Compartment
mitochondrial matrix
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(S)-Malate + NAD+ <=> Oxaloacetate + NADH + H+
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The layout of this reaction may differ from that in the pathway view due to the constraints in pathway layout

Mitochondrial malate dehydrogenase catalyzes the reversible reaction of malate and NAD+ to form oxaloacetate (OA) and NADH + H+ (Luo et al. 2006). This reaction is highly endergonic but is pulled in the direction annotated here when the TCA cycle is operating. Unpublished crystallographic data indicate that the protein is a dimer (PDB 3E04).

Literature References
PubMed ID Title Journal Year
16740313 An NADH-tetrazolium-coupled sensitive assay for malate dehydrogenase in mitochondria and crude tissue homogenates

Liu, J, Wang, X, Long, J, Luo, C

J Biochem Biophys Methods 2006
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Event Information
Reverse Reaction
MDH2 reduces OA to MAL (Homo sapiens)
Catalyst Activity

L-malate dehydrogenase activity of MDH2 dimer [mitochondrial matrix]

Physical Entity
MDH2 dimer [mitochondrial matrix] (Homo sapiens)
Activity
L-malate dehydrogenase activity (GO:0030060)
Orthologous Events
malate + NAD+ <=> oxaloacetate + NADH + H+ (Gallus gallus)
(S)-Malate + NAD+ <=> Oxaloacetate + NADH + H+ (Bos taurus)
(S)-Malate + NAD+ <=> Oxaloacetate + NADH + H+ (Caenorhabditis elegans)
(S)-Malate + NAD+ <=> Oxaloacetate + NADH + H+ (Canis familiaris)
(S)-Malate + NAD+ <=> Oxaloacetate + NADH + H+ (Danio rerio)
(S)-Malate + NAD+ <=> Oxaloacetate + NADH + H+ (Drosophila melanogaster)
(S)-Malate + NAD+ <=> Oxaloacetate + NADH + H+ (Gallus gallus)
(S)-Malate + NAD+ <=> Oxaloacetate + NADH + H+ (Mus musculus)
(S)-Malate + NAD+ <=> Oxaloacetate + NADH + H+ (Plasmodium falciparum)
(S)-Malate + NAD+ <=> Oxaloacetate + NADH + H+ (Rattus norvegicus)
(S)-Malate + NAD+ <=> Oxaloacetate + NADH + H+ (Saccharomyces cerevisiae)
(S)-Malate + NAD+ <=> Oxaloacetate + NADH + H+ (Schizosaccharomyces pombe)
(S)-Malate + NAD+ <=> Oxaloacetate + NADH + H+ (Sus scrofa)
(S)-Malate + NAD+ <=> Oxaloacetate + NADH + H+ (Xenopus tropicalis)
Cross References
Rhea
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