Reactome | isocitrate + NAD+ => alpha-ketoglutarate + CO2 + NADH + H+ [IDH3]

isocitrate + NAD+ => alpha-ketoglutarate + CO2 + NADH + H+ [IDH3]

Stable Identifier

R-HSA-70967

Type

Reaction [transition]

Species

Homo sapiens

Compartment

mitochondrial matrix

Locations in the PathwayBrowser

Expand all

General

SBML | | PDF

SVG | | PPTX | SBGN

isocitrate + NAD+ => alpha-ketoglutarate + CO2 + NADH + H+ [IDH3]

Click the image above or here to open this reaction in the Pathway Browser
The layout of this reaction may differ from that in the pathway view due to the constraints in pathway layout

Mitochondrial isocitrate dehydrogenase IDH3 catalyzes the irreversible reaction of isocitrate and NAD+ to form alpha ketoglutarate, CO2, and NADH + H+. The enzyme is a heteromer containing four polypeptide chains, two IDH3A, one IDH3B, and one IDH3G, and two Mn++ (Dange and Colman 2010). It is activated by ADP (Soundar et al. 2003, 2006; Bzymek and Colman 2007). This is the first of four oxidation reactions in the citric acid cycle, and the first decarboxylation.

Literature References

PubMed ID	Title	Journal	Year
17432878	Role of alpha-Asp181, beta-Asp192, and gamma-Asp190 in the distinctive subunits of human NAD-specific isocitrate dehydrogenase Bzymek, KP, Colman, RF	Biochemistry	2007
16737955	Identification of Mn2+-binding aspartates from alpha, beta, and gamma subunits of human NAD-dependent isocitrate dehydrogenase Soundar, S, O'Hagan, M, Fomulu, KS, Colman, RF	J Biol Chem	2006
20435888	Each conserved active site tyr in the three subunits of human isocitrate dehydrogenase has a different function Dange, M, Colman, RF	J Biol Chem	2010
14555658	Evaluation by mutagenesis of the importance of 3 arginines in alpha, beta, and gamma subunits of human NAD-dependent isocitrate dehydrogenase Soundar, S, Park, JH, Colman, RF	J Biol Chem	2003